Abstract
We studied the metallothionein (MT) induction by cadmium (Cd) in the dental pulp and bone in rats. In order to clarify the cell response to Cd in the hard tissue, the isoform-specific expression of MT mRNAs (MT-I and MT-II) was examined by reverse transcriptase-polymerase chain reaction (RT-PCR) method, and the localization of MT was confirmed by immunohistochemical studies. Both MT-I and MT-II mRNA levels were increased within 3 hours by Cd administration in both the dental pulp and bone. In the dental pulp, MT protein was localized in the specific cell type of odontoblasts (secretory odontoblasts and resting odontoblasts). In the bone, MT-positive cells were time-dependently increased, and the positive cells were mainly localized in osteocytes. The cell-specific induction of MT may be associated with Cd accumulation and Cd-induced the hard tissue injury in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 269-270 |
| Number of pages | 2 |
| Journal | Journal of Hard Tissue Biology |
| Volume | 14 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2005 |
| Externally published | Yes |
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Keywords
- Cadmium
- Metallothionein
- Odontblast
- Osteoclast
- Osteocyte
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Biochemistry
- Biomaterials
- Orthopedics and Sports Medicine
- Dentistry(all)
- Cell Biology
Cite this
The Role of Metallothionein in Hard Tissue. / Sogawa, Norio; Sogawa, Chiharu; Onodera, Kenji; Oda, Nariaki; Furuta, Hiroaki; Kitayama, Shigeo; Yamamoto, Toshio.
In: Journal of Hard Tissue Biology, Vol. 14, No. 2, 2005, p. 269-270.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Role of Metallothionein in Hard Tissue
AU - Sogawa, Norio
AU - Sogawa, Chiharu
AU - Onodera, Kenji
AU - Oda, Nariaki
AU - Furuta, Hiroaki
AU - Kitayama, Shigeo
AU - Yamamoto, Toshio
PY - 2005
Y1 - 2005
N2 - We studied the metallothionein (MT) induction by cadmium (Cd) in the dental pulp and bone in rats. In order to clarify the cell response to Cd in the hard tissue, the isoform-specific expression of MT mRNAs (MT-I and MT-II) was examined by reverse transcriptase-polymerase chain reaction (RT-PCR) method, and the localization of MT was confirmed by immunohistochemical studies. Both MT-I and MT-II mRNA levels were increased within 3 hours by Cd administration in both the dental pulp and bone. In the dental pulp, MT protein was localized in the specific cell type of odontoblasts (secretory odontoblasts and resting odontoblasts). In the bone, MT-positive cells were time-dependently increased, and the positive cells were mainly localized in osteocytes. The cell-specific induction of MT may be associated with Cd accumulation and Cd-induced the hard tissue injury in vivo.
AB - We studied the metallothionein (MT) induction by cadmium (Cd) in the dental pulp and bone in rats. In order to clarify the cell response to Cd in the hard tissue, the isoform-specific expression of MT mRNAs (MT-I and MT-II) was examined by reverse transcriptase-polymerase chain reaction (RT-PCR) method, and the localization of MT was confirmed by immunohistochemical studies. Both MT-I and MT-II mRNA levels were increased within 3 hours by Cd administration in both the dental pulp and bone. In the dental pulp, MT protein was localized in the specific cell type of odontoblasts (secretory odontoblasts and resting odontoblasts). In the bone, MT-positive cells were time-dependently increased, and the positive cells were mainly localized in osteocytes. The cell-specific induction of MT may be associated with Cd accumulation and Cd-induced the hard tissue injury in vivo.
KW - Cadmium
KW - Metallothionein
KW - Odontblast
KW - Osteoclast
KW - Osteocyte
UR - http://www.scopus.com/inward/record.url?scp=85024751857&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85024751857&partnerID=8YFLogxK
U2 - 10.2485/jhtb.14.269
DO - 10.2485/jhtb.14.269
M3 - Article
VL - 14
SP - 269
EP - 270
JO - Journal of Hard Tissue Biology
JF - Journal of Hard Tissue Biology
SN - 1341-7649
IS - 2
ER -