The role of membrane potential for the control of elongation growth of vigna hypocotyl: Response of a hollow cylinder to osmotic and ionic stress

Kiyoshi Nakahori, Kiyoshi Katou, Hisashi Okamoto

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We have devised an experimental system of perfusion through a hollow cylinder of a Vigna hypocotyl to examine the control mechanism of plant stem elongation. When the cylinder was subjected to osmotic stress, it began to shrink and then spontaneously resumed elongation. Not only the membrane potential difference between the parenchyma symplast and the central bore (Vpx), but also that between the parenchyma symplast and the organ surface (Vps), showed hyperpolarization a few minutes after the cylinder began to shrink. Removal of the stress caused an immediate increase in elongation rate followed by depolarization of both membrane potentials a few minutes later. When the cylinder was subjected to KCl stress, Vpx showed transient depolarization and recovery, while Vps showed only immediate hyperpolarization. Increasing the KCl concentration caused Vpx to depolarize, and the cylinder simultaneously to cease to elongate for about 5min, even when the osmotic concentration of the perfusion solution was kept almost constant. An inverse reaction was observed when the KCl concentration was decreased.These two reversible responses suggest that control of Vpx may regulate the elongation of hollow cylinders, and that the xylem pump plays an important role in the regulation of intact stem elongation.

Original languageEnglish
Pages (from-to)901-910
Number of pages10
JournalPlant and Cell Physiology
Volume28
Issue number5
Publication statusPublished - Jul 1987
Externally publishedYes

Fingerprint

Hypocotyl
symplast
Vigna
Membrane Potential
Osmotic Pressure
stem elongation
Elongation
membrane potential
Membrane Potentials
hypocotyls
parenchyma (plant tissue)
Perfusion
Plant Stems
membrane
Membranes
Xylem
Growth
osmotic stress
pumps
xylem

Keywords

  • Electrogenic ion pump
  • Hollow cylinder (hypocotyl)
  • Ionic stress
  • Osmotic stress
  • Perfusion
  • Vigna unguiculata

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

The role of membrane potential for the control of elongation growth of vigna hypocotyl : Response of a hollow cylinder to osmotic and ionic stress. / Nakahori, Kiyoshi; Katou, Kiyoshi; Okamoto, Hisashi.

In: Plant and Cell Physiology, Vol. 28, No. 5, 07.1987, p. 901-910.

Research output: Contribution to journalArticle

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