The role of hybrid chitosan membranes on scarring process following lumbar surgery

Post-laminectomy experimental model

Miguel Carvalho, Luís M. Costa, José E. Pereira, Yuki Shirosaki, Satoshi Hayakawa, José D. Santos, Stefano Geuna, Federica Fregnan, António M. Cabrita, Ana C. Maurício, Artur S. Varejão

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objectives: Post-operative scarring process on lumbar surgery is object of several studies mainly because of the epidural fibrosis formation. Hybrid chitosan have shown promising effect on fibrosis prevention. The aim of this study was to determine the influence of chitosan-silane membrane on the lumbar surgery scarring process. These membranes have improved mechanical strength which makes them suitable to maintain a predefined shape.

Methods: A two level lumbar laminectomy was performed in 14 New Zealand male rabbits. Laminectomy sites were randomly selected for biomaterial or control. Chitosan membranes were prepared and care was taken in order to make it adapted to the bone defect dimensions covering the totality of the defect including the bone margins. Histological analysis was performed by haematoxylin/eosin and by Masson’s trichrome staining four weeks after laminectomy.

Results: Microscope observations revealed the presence of a well-organized regenerating tissue, integrated in the surrounding vertebral bone tissue with a regular and all-site interface on the chitosan sites, in clear contrast with the presence of a disorganized regenerating tissue with aspects consistent with the persistence of a chronic inflammatory condition, on control sites.

Discussion: The results of this study clearly demonstrated that hybrid chitosan had an organizing effect on post-operative scarring process. The presence of the hybrid chitosan membrane resulted on a wellorganized tissue integrated in the surrounding vertebral bone tissue with signs of regenerative bone tissue in continuity with native bone. This can be a major feature on the dynamics of epidural fibrosis formation.

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalNeurological Research
Volume37
Issue number1
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Laminectomy
Chitosan
Cicatrix
Theoretical Models
Bone and Bones
Membranes
Fibrosis
Silanes
Biocompatible Materials
Hematoxylin
Eosine Yellowish-(YS)
Staining and Labeling
Rabbits

Keywords

  • Chitosan-silane
  • Epidural fibrosis
  • Hybrid chitosan
  • Laminectomy
  • Lumbar surgery

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology
  • Medicine(all)

Cite this

The role of hybrid chitosan membranes on scarring process following lumbar surgery : Post-laminectomy experimental model. / Carvalho, Miguel; Costa, Luís M.; Pereira, José E.; Shirosaki, Yuki; Hayakawa, Satoshi; Santos, José D.; Geuna, Stefano; Fregnan, Federica; Cabrita, António M.; Maurício, Ana C.; Varejão, Artur S.

In: Neurological Research, Vol. 37, No. 1, 01.01.2015, p. 23-29.

Research output: Contribution to journalArticle

Carvalho, M, Costa, LM, Pereira, JE, Shirosaki, Y, Hayakawa, S, Santos, JD, Geuna, S, Fregnan, F, Cabrita, AM, Maurício, AC & Varejão, AS 2015, 'The role of hybrid chitosan membranes on scarring process following lumbar surgery: Post-laminectomy experimental model', Neurological Research, vol. 37, no. 1, pp. 23-29. https://doi.org/10.1179/1743132814Y.0000000414
Carvalho, Miguel ; Costa, Luís M. ; Pereira, José E. ; Shirosaki, Yuki ; Hayakawa, Satoshi ; Santos, José D. ; Geuna, Stefano ; Fregnan, Federica ; Cabrita, António M. ; Maurício, Ana C. ; Varejão, Artur S. / The role of hybrid chitosan membranes on scarring process following lumbar surgery : Post-laminectomy experimental model. In: Neurological Research. 2015 ; Vol. 37, No. 1. pp. 23-29.
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