The role of glucocorticoid in the regulation of prostaglandin biosynthesis in non-pregnant bovine endometrium

Hwa Yong Lee, Tomas J. Acosta, Michiyo Tanikawa, Ryosuke Sakumoto, Junichi Komiyama, Yukari Tasaki, Mariusz Piskula, Dariusz J. Skarzynski, Masafumi Tetsuka, Kiyoshi Okuda

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

To determine whether glucocorticoids (GCs) play a role in regulating uterine function in cow, the present study examined the expression of mRNA encoding GC receptor (GC-R) α, 11β-hydroxysteroid dehydrogenase (11-HSD) type 1 and type 2, and the activity of 11-HSD1 in bovine endometrial tissue throughout the estrous cycle. We also studied the effects of cortisol on basal, oxytocin (OT)- and tumor necrosis factor-α (TNFα)-stimulated prostaglandin (PG) production. A quantitative real-time PCR analysis revealed that GC-Rα mRNA was expressed more strongly in the mid-luteal stage (days 8-12) than in the other stages. In contrast to GC-Rα mRNA expression, 11-HSD1 mRNA expression was greater in the follicular stage than in the other stages, whereas 11-HSD2 mRNA expression was lowest in the follicular stage. The activity of 11-HSD1 was greater in the follicular stage and estrus than in the other stages and was lowest in the mid-luteal stage. Cortisone was dose-dependently converted to cortisol in the cultured endometrial tissue. Although cortisol did not affect either the basal or OT-stimulated production of PGs in the cultured epithelial cells, the production of PGs stimulated by TNFα in the stromal cells was suppressed by cortisol (P

Original languageEnglish
Pages (from-to)127-135
Number of pages9
JournalJournal of Endocrinology
Volume193
Issue number1
DOIs
Publication statusPublished - Apr 2007

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Endometrium
Glucocorticoids
Prostaglandins
Hydrocortisone
Messenger RNA
Corpus Luteum
Oxytocin
Tumor Necrosis Factor-alpha
11-beta-Hydroxysteroid Dehydrogenases
Estrous Cycle
Cortisone
Glucocorticoid Receptors
Estrus
Stromal Cells
Real-Time Polymerase Chain Reaction
Cultured Cells
Epithelial Cells

ASJC Scopus subject areas

  • Endocrinology

Cite this

Lee, H. Y., Acosta, T. J., Tanikawa, M., Sakumoto, R., Komiyama, J., Tasaki, Y., ... Okuda, K. (2007). The role of glucocorticoid in the regulation of prostaglandin biosynthesis in non-pregnant bovine endometrium. Journal of Endocrinology, 193(1), 127-135. https://doi.org/10.1677/joe.1.06975

The role of glucocorticoid in the regulation of prostaglandin biosynthesis in non-pregnant bovine endometrium. / Lee, Hwa Yong; Acosta, Tomas J.; Tanikawa, Michiyo; Sakumoto, Ryosuke; Komiyama, Junichi; Tasaki, Yukari; Piskula, Mariusz; Skarzynski, Dariusz J.; Tetsuka, Masafumi; Okuda, Kiyoshi.

In: Journal of Endocrinology, Vol. 193, No. 1, 04.2007, p. 127-135.

Research output: Contribution to journalArticle

Lee, HY, Acosta, TJ, Tanikawa, M, Sakumoto, R, Komiyama, J, Tasaki, Y, Piskula, M, Skarzynski, DJ, Tetsuka, M & Okuda, K 2007, 'The role of glucocorticoid in the regulation of prostaglandin biosynthesis in non-pregnant bovine endometrium', Journal of Endocrinology, vol. 193, no. 1, pp. 127-135. https://doi.org/10.1677/joe.1.06975
Lee, Hwa Yong ; Acosta, Tomas J. ; Tanikawa, Michiyo ; Sakumoto, Ryosuke ; Komiyama, Junichi ; Tasaki, Yukari ; Piskula, Mariusz ; Skarzynski, Dariusz J. ; Tetsuka, Masafumi ; Okuda, Kiyoshi. / The role of glucocorticoid in the regulation of prostaglandin biosynthesis in non-pregnant bovine endometrium. In: Journal of Endocrinology. 2007 ; Vol. 193, No. 1. pp. 127-135.
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