The role of glucocorticoid in the regulation of prostaglandin biosynthesis in non-pregnant bovine endometrium

Hwa Yong Lee, Tomas J. Acosta, Michiyo Tanikawa, Ryosuke Sakumoto, Junichi Komiyama, Yukari Tasaki, Mariusz Piskula, Dariusz J. Skarzynski, Masafumi Tetsuka, Kiyoshi Okuda

Research output: Contribution to journalArticle

28 Citations (Scopus)


To determine whether glucocorticoids (GCs) play a role in regulating uterine function in cow, the present study examined the expression of mRNA encoding GC receptor (GC-R) α, 11β-hydroxysteroid dehydrogenase (11-HSD) type 1 and type 2, and the activity of 11-HSD1 in bovine endometrial tissue throughout the estrous cycle. We also studied the effects of cortisol on basal, oxytocin (OT)- and tumor necrosis factor-α (TNFα)-stimulated prostaglandin (PG) production. A quantitative real-time PCR analysis revealed that GC-Rα mRNA was expressed more strongly in the mid-luteal stage (days 8-12) than in the other stages. In contrast to GC-Rα mRNA expression, 11-HSD1 mRNA expression was greater in the follicular stage than in the other stages, whereas 11-HSD2 mRNA expression was lowest in the follicular stage. The activity of 11-HSD1 was greater in the follicular stage and estrus than in the other stages and was lowest in the mid-luteal stage. Cortisone was dose-dependently converted to cortisol in the cultured endometrial tissue. Although cortisol did not affect either the basal or OT-stimulated production of PGs in the cultured epithelial cells, the production of PGs stimulated by TNFα in the stromal cells was suppressed by cortisol (P

Original languageEnglish
Pages (from-to)127-135
Number of pages9
JournalJournal of Endocrinology
Issue number1
Publication statusPublished - Apr 2007


ASJC Scopus subject areas

  • Endocrinology

Cite this

Lee, H. Y., Acosta, T. J., Tanikawa, M., Sakumoto, R., Komiyama, J., Tasaki, Y., Piskula, M., Skarzynski, D. J., Tetsuka, M., & Okuda, K. (2007). The role of glucocorticoid in the regulation of prostaglandin biosynthesis in non-pregnant bovine endometrium. Journal of Endocrinology, 193(1), 127-135.