Abstract
A successful pregnancy depends on the blastocyst's implantation to the maternal endometrium; however, the initial interaction between blastocyst and uterine epithelium has not been well characterized. The objectives of this study were to determine if selectins and their ligands were expressed in the bovine conceptus and/or uterus during the periattachment period and to study whether selectins were associated with conceptus attachment to the uterine epithelium. Through the RNA-sequence analysis of bovine conceptuses on Days 17, 20, and 22 (Day 0=day of estrus), only the SELL ligand, podocalyxin (PODXL), and P-selectin (SELP) ligand, SELPLG, were found. Quantitative PCR analysis confirmed the presence of PODXL and SELPLG in these conceptuses and revealed that SELL, mRNA and protein, detected in the uterine epithelium but not in conceptuses increased during the periattachment period. In the cultured endometrial epithelial cells (EECs), SELL transcript was up-regulated when uterine flushings from Day 20 pregnant animals were placed onto these cells. SELL was also up-regulated when cultured EECs were treated with progesterone, EGF, or bFGF, but not with IFNT. In the coculture system with EECs and bovine trophoblast CT-1 cells, SELL expression in EECs was effectively reduced by its small interfering RNA; however, IFNT, a marker for CT-1 cell attachment to EECs, was not reduced, nor was a transcription factor of IFNT, CDX2. These observations suggest that the conceptus could attach to the uterine epithelium through the use of endometrial SELL and embryonic selectin ligands, possibly initiating the conceptus attachment process in the bovine species.
| Original language | English |
|---|---|
| Article number | 46 |
| Journal | Biology of Reproduction |
| Volume | 93 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 1 2015 |
Fingerprint
Keywords
- Attachment
- Bovine
- Coculture
- Conceptus
- Selectin
ASJC Scopus subject areas
- Cell Biology
Cite this
The role of endometrial selectins and their ligands on bovine conceptus attachment to the uterine epithelium during peri-implantation period. / Bai, Rulan; Kusama, Kazuya; Sakurai, Toshihiro; Bai, Hanako; Wang, Changshou; Zhang, Jinfeng; Kuse, Mariko; Ideta, Atsushi; Aoyagi, Yoshito; Okuda, Kiyoshi; Imakawa, Kazuhiko.
In: Biology of Reproduction, Vol. 93, No. 2, 46, 01.08.2015.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The role of endometrial selectins and their ligands on bovine conceptus attachment to the uterine epithelium during peri-implantation period
AU - Bai, Rulan
AU - Kusama, Kazuya
AU - Sakurai, Toshihiro
AU - Bai, Hanako
AU - Wang, Changshou
AU - Zhang, Jinfeng
AU - Kuse, Mariko
AU - Ideta, Atsushi
AU - Aoyagi, Yoshito
AU - Okuda, Kiyoshi
AU - Imakawa, Kazuhiko
PY - 2015/8/1
Y1 - 2015/8/1
N2 - A successful pregnancy depends on the blastocyst's implantation to the maternal endometrium; however, the initial interaction between blastocyst and uterine epithelium has not been well characterized. The objectives of this study were to determine if selectins and their ligands were expressed in the bovine conceptus and/or uterus during the periattachment period and to study whether selectins were associated with conceptus attachment to the uterine epithelium. Through the RNA-sequence analysis of bovine conceptuses on Days 17, 20, and 22 (Day 0=day of estrus), only the SELL ligand, podocalyxin (PODXL), and P-selectin (SELP) ligand, SELPLG, were found. Quantitative PCR analysis confirmed the presence of PODXL and SELPLG in these conceptuses and revealed that SELL, mRNA and protein, detected in the uterine epithelium but not in conceptuses increased during the periattachment period. In the cultured endometrial epithelial cells (EECs), SELL transcript was up-regulated when uterine flushings from Day 20 pregnant animals were placed onto these cells. SELL was also up-regulated when cultured EECs were treated with progesterone, EGF, or bFGF, but not with IFNT. In the coculture system with EECs and bovine trophoblast CT-1 cells, SELL expression in EECs was effectively reduced by its small interfering RNA; however, IFNT, a marker for CT-1 cell attachment to EECs, was not reduced, nor was a transcription factor of IFNT, CDX2. These observations suggest that the conceptus could attach to the uterine epithelium through the use of endometrial SELL and embryonic selectin ligands, possibly initiating the conceptus attachment process in the bovine species.
AB - A successful pregnancy depends on the blastocyst's implantation to the maternal endometrium; however, the initial interaction between blastocyst and uterine epithelium has not been well characterized. The objectives of this study were to determine if selectins and their ligands were expressed in the bovine conceptus and/or uterus during the periattachment period and to study whether selectins were associated with conceptus attachment to the uterine epithelium. Through the RNA-sequence analysis of bovine conceptuses on Days 17, 20, and 22 (Day 0=day of estrus), only the SELL ligand, podocalyxin (PODXL), and P-selectin (SELP) ligand, SELPLG, were found. Quantitative PCR analysis confirmed the presence of PODXL and SELPLG in these conceptuses and revealed that SELL, mRNA and protein, detected in the uterine epithelium but not in conceptuses increased during the periattachment period. In the cultured endometrial epithelial cells (EECs), SELL transcript was up-regulated when uterine flushings from Day 20 pregnant animals were placed onto these cells. SELL was also up-regulated when cultured EECs were treated with progesterone, EGF, or bFGF, but not with IFNT. In the coculture system with EECs and bovine trophoblast CT-1 cells, SELL expression in EECs was effectively reduced by its small interfering RNA; however, IFNT, a marker for CT-1 cell attachment to EECs, was not reduced, nor was a transcription factor of IFNT, CDX2. These observations suggest that the conceptus could attach to the uterine epithelium through the use of endometrial SELL and embryonic selectin ligands, possibly initiating the conceptus attachment process in the bovine species.
KW - Attachment
KW - Bovine
KW - Coculture
KW - Conceptus
KW - Selectin
UR - http://www.scopus.com/inward/record.url?scp=84941568174&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941568174&partnerID=8YFLogxK
U2 - 10.1095/biolreprod.115.128652
DO - 10.1095/biolreprod.115.128652
M3 - Article
AN - SCOPUS:84941568174
VL - 93
JO - Biology of Reproduction
JF - Biology of Reproduction
SN - 0006-3363
IS - 2
M1 - 46
ER -