The role of dopamine in manganese-induced oxidative injury in rat pheochromocytoma cells

K. Seth, A. K. Agrawal, Isao Date, P. K. Seth

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Reactive dopamine (DA) metabolites have been implicated in both Parkinson's disease and manganese (Mn) neurotoxicity. Rat PC12 and genetically modified PC12 (PC12M) cells capable of producing higher DA content, on exposure to MnCl2 (10-6 M) for 72 hours, exhibited a significant decrease in glutathione content. Activity of antioxidant enzyme catalase was also inhibited following 24- and 72-hour MnCl2 exposure. MnCl2 caused a concentration-dependent (10-7 to 10-3 M) loss in mitochondrial activity after 24 and 72 hours and an impaired DNA synthesis after 72 hours with changes being more marked in PC12M cells. The results indicate that the free-radical-mediated toxicity of Mn at cellular level involves down-regulation of anti-oxidants in normal and DA-rich PC12 cells. PC12M cells appeared to be more sensitive than PC12 cells.

Original languageEnglish
Pages (from-to)165-170
Number of pages6
JournalHuman and Experimental Toxicology
Volume21
Issue number3
DOIs
Publication statusPublished - 2002

Fingerprint

PC12 Cells
Pheochromocytoma
Manganese
Rats
Dopamine
Wounds and Injuries
Metabolites
Oxidants
Catalase
Free Radicals
Glutathione
Parkinson Disease
Toxicity
Down-Regulation
Antioxidants
DNA
Enzymes
manganese chloride

Keywords

  • Catalase
  • Dopamine
  • Glutathione
  • Mn
  • PC12 cell line
  • SOD

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

The role of dopamine in manganese-induced oxidative injury in rat pheochromocytoma cells. / Seth, K.; Agrawal, A. K.; Date, Isao; Seth, P. K.

In: Human and Experimental Toxicology, Vol. 21, No. 3, 2002, p. 165-170.

Research output: Contribution to journalArticle

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