The role of dopamine in manganese-induced oxidative injury in rat pheochromocytoma cells

K. Seth, A. K. Agrawal, I. Date, P. K. Seth

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Reactive dopamine (DA) metabolites have been implicated in both Parkinson's disease and manganese (Mn) neurotoxicity. Rat PC12 and genetically modified PC12 (PC12M) cells capable of producing higher DA content, on exposure to MnCl2 (10-6 M) for 72 hours, exhibited a significant decrease in glutathione content. Activity of antioxidant enzyme catalase was also inhibited following 24- and 72-hour MnCl2 exposure. MnCl2 caused a concentration-dependent (10-7 to 10-3 M) loss in mitochondrial activity after 24 and 72 hours and an impaired DNA synthesis after 72 hours with changes being more marked in PC12M cells. The results indicate that the free-radical-mediated toxicity of Mn at cellular level involves down-regulation of anti-oxidants in normal and DA-rich PC12 cells. PC12M cells appeared to be more sensitive than PC12 cells.

Original languageEnglish
Pages (from-to)165-170
Number of pages6
JournalHuman and Experimental Toxicology
Volume21
Issue number3
DOIs
Publication statusPublished - Jun 15 2002

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Keywords

  • Catalase
  • Dopamine
  • Glutathione
  • Mn
  • PC12 cell line
  • SOD

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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