Abstract
Multipotential ability of bone marrow-derived cells has been clarified, and their involvement in repair and maintenance of various tissues has been reported. However, the role of bone marrow-derived cells in osteogenesis remains unknown. In the present study, bone marrow-derived cells during ectopic bone formation of mouse femoral muscle were traced using a GFP bone marrow transplantation model. Bone marrow cells from C57BL/6-Tg (CAG-EGFP) mice were transplanted into C57BL/6 J wild type mice. After transplantation, insoluble bone matrix (IBM) was implanted into mouse muscle. Ectopic bone formation was histologically assessed at postoperative days 7, 14, and 28. Immunohistochemistry for GFP single staining and GFP-osteocalcin double staining was then performed. Bone marrow transplantation successfully replaced hematopoietic cells with GFP-positive donor cells. Immunohistochemical analyses revealed that osteoblasts and osteocytes involved in ectopic bone formation were GFP-negative, whereas osteoclasts and hematopoietic cells involved in bone formation were GFP-positive. These results indicate that bone marrow-derived cells might not differentiate into osteoblasts. Thus, the main role of bone marrow-derived cells in ectopic osteogenesis may not be to induce bone regeneration by differentiation into osteoblasts, but rather to contribute to microenvironment formation for bone formation by differentiating tissue stem cells into osteoblasts.
| Original language | English |
|---|---|
| Pages (from-to) | 748-757 |
| Number of pages | 10 |
| Journal | International Journal of Medical Sciences |
| Volume | 15 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - May 22 2018 |
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Keywords
- Bone marrow transplantation
- Bone marrow-derived cell
- Ectopic bone formation
- GFP
- Insoluble bone matrix (IBM)
- Osteoblast
ASJC Scopus subject areas
- Medicine(all)
Cite this
The role of bone marrow-derived cells during ectopic bone formation of mouse femoral muscle in GFP mouse bone marrow transplantation model. / Takabatake, Kiyofumi; Tsujigiwa, Hidetsugu; Song, Yu; Matsuda, Hiroyuki; Kawai, Hotaka; Fujii, Masae; Hamada, Mei; Nakano, Keisuke; Kawakami, Toshiyuki; Nagatsuka, Hitoshi.
In: International Journal of Medical Sciences, Vol. 15, No. 8, 22.05.2018, p. 748-757.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The role of bone marrow-derived cells during ectopic bone formation of mouse femoral muscle in GFP mouse bone marrow transplantation model
AU - Takabatake, Kiyofumi
AU - Tsujigiwa, Hidetsugu
AU - Song, Yu
AU - Matsuda, Hiroyuki
AU - Kawai, Hotaka
AU - Fujii, Masae
AU - Hamada, Mei
AU - Nakano, Keisuke
AU - Kawakami, Toshiyuki
AU - Nagatsuka, Hitoshi
PY - 2018/5/22
Y1 - 2018/5/22
N2 - Multipotential ability of bone marrow-derived cells has been clarified, and their involvement in repair and maintenance of various tissues has been reported. However, the role of bone marrow-derived cells in osteogenesis remains unknown. In the present study, bone marrow-derived cells during ectopic bone formation of mouse femoral muscle were traced using a GFP bone marrow transplantation model. Bone marrow cells from C57BL/6-Tg (CAG-EGFP) mice were transplanted into C57BL/6 J wild type mice. After transplantation, insoluble bone matrix (IBM) was implanted into mouse muscle. Ectopic bone formation was histologically assessed at postoperative days 7, 14, and 28. Immunohistochemistry for GFP single staining and GFP-osteocalcin double staining was then performed. Bone marrow transplantation successfully replaced hematopoietic cells with GFP-positive donor cells. Immunohistochemical analyses revealed that osteoblasts and osteocytes involved in ectopic bone formation were GFP-negative, whereas osteoclasts and hematopoietic cells involved in bone formation were GFP-positive. These results indicate that bone marrow-derived cells might not differentiate into osteoblasts. Thus, the main role of bone marrow-derived cells in ectopic osteogenesis may not be to induce bone regeneration by differentiation into osteoblasts, but rather to contribute to microenvironment formation for bone formation by differentiating tissue stem cells into osteoblasts.
AB - Multipotential ability of bone marrow-derived cells has been clarified, and their involvement in repair and maintenance of various tissues has been reported. However, the role of bone marrow-derived cells in osteogenesis remains unknown. In the present study, bone marrow-derived cells during ectopic bone formation of mouse femoral muscle were traced using a GFP bone marrow transplantation model. Bone marrow cells from C57BL/6-Tg (CAG-EGFP) mice were transplanted into C57BL/6 J wild type mice. After transplantation, insoluble bone matrix (IBM) was implanted into mouse muscle. Ectopic bone formation was histologically assessed at postoperative days 7, 14, and 28. Immunohistochemistry for GFP single staining and GFP-osteocalcin double staining was then performed. Bone marrow transplantation successfully replaced hematopoietic cells with GFP-positive donor cells. Immunohistochemical analyses revealed that osteoblasts and osteocytes involved in ectopic bone formation were GFP-negative, whereas osteoclasts and hematopoietic cells involved in bone formation were GFP-positive. These results indicate that bone marrow-derived cells might not differentiate into osteoblasts. Thus, the main role of bone marrow-derived cells in ectopic osteogenesis may not be to induce bone regeneration by differentiation into osteoblasts, but rather to contribute to microenvironment formation for bone formation by differentiating tissue stem cells into osteoblasts.
KW - Bone marrow transplantation
KW - Bone marrow-derived cell
KW - Ectopic bone formation
KW - GFP
KW - Insoluble bone matrix (IBM)
KW - Osteoblast
UR - http://www.scopus.com/inward/record.url?scp=85049473933&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85049473933&partnerID=8YFLogxK
U2 - 10.7150/ijms.24605
DO - 10.7150/ijms.24605
M3 - Article
C2 - 30008583
AN - SCOPUS:85049473933
VL - 15
SP - 748
EP - 757
JO - International Journal of Medical Sciences
JF - International Journal of Medical Sciences
SN - 1449-1907
IS - 8
ER -