TY - JOUR
T1 - The rice wound-inducible transcription factor RERJ1 sharing same signal transduction pathway with OsMYC2 is necessary for defense response to herbivory and bacterial blight
AU - Valea, Ioana
AU - Motegi, Atsushi
AU - Kawamura, Naoko
AU - Kawamoto, Koichi
AU - Miyao, Akio
AU - Ozawa, Rika
AU - Takabayashi, Junji
AU - Gomi, Kenji
AU - Nemoto, Keiichirou
AU - Nozawa, Akira
AU - Sawasaki, Tatsuya
AU - Shinya, Tomonori
AU - Galis, Ivan
AU - Miyamoto, Koji
AU - Nojiri, Hideaki
AU - Okada, Kazunori
N1 - Funding Information:
This study was partially supported by JSPS KAKENHI (Grant Numbers 17H03811 and 20H02922 to KO), Japan-Austria Research Cooperative Program between JSPS and FWF (Grant Number JPJSBP120202002 to KO), and the Joint Usage/Research Center, Institute of Plant Science and Resources, Okayama University.
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Nature B.V.
PY - 2021
Y1 - 2021
N2 - Key message: This study describes biological functions of the bHLH transcription factor RERJ1 involved in the jasmonate response and the related defense-associated metabolic pathways in rice, with particular focus on deciphering the regulatory mechanisms underlying stress-induced volatile emission and herbivory resistance. RERJ1 is rapidly and drastically induced by wounding and jasmonate treatment but its biological function remains unknown as yet. Here we provide evidence of the biological function of RERJ1 in plant defense, specifically in response to herbivory and pathogen attack, and offer insights into the RERJ1-mediated regulation of metabolic pathways of specialized defense compounds, such as monoterpene linalool, in possible collaboration with OsMYC2—a well-known master regulator in jasmonate signaling. Abstract: In rice (Oryza sativa L.), the basic helix–loop–helix (bHLH) family transcription factor RERJ1 is induced under environmental stresses, such as wounding and drought, which are closely linked to jasmonate (JA) accumulation. Here, we investigated the biological function of RERJ1 in response to biotic stresses, such as herbivory and pathogen infection, using an RERJ1-defective mutant. Transcriptome analysis of the rerj1-Tos17 mutant revealed that RERJ1 regulated the expression of a typical family of conserved JA-responsive genes (e.g., terpene synthases, proteinase inhibitors, and jasmonate ZIM domain proteins). Upon exposure to armyworm attack, the rerj1-Tos17 mutant exhibited more severe damage than the wildtype, and significant weight gain of the larvae fed on the mutant was observed. Upon Xanthomonas oryzae infection, the rerj1-Tos17 mutant developed more severe symptoms than the wildtype. Among RERJ1-regulated terpene synthases, linalool synthase expression was markedly disrupted and linalool emission after wounding was significantly decreased in the rerj1-Tos17 mutant. RERJ1 appears to interact with OsMYC2—a master regulator of JA signaling—and many OsJAZ proteins, although no obvious epistatic interaction was detected between them at the transcriptional level. These results indicate that RERJ1 is involved in the transcriptional induction of JA-mediated stress-responsive genes via physical association with OsMYC2 and mediates defense against herbivory and bacterial infection through JA signaling.
AB - Key message: This study describes biological functions of the bHLH transcription factor RERJ1 involved in the jasmonate response and the related defense-associated metabolic pathways in rice, with particular focus on deciphering the regulatory mechanisms underlying stress-induced volatile emission and herbivory resistance. RERJ1 is rapidly and drastically induced by wounding and jasmonate treatment but its biological function remains unknown as yet. Here we provide evidence of the biological function of RERJ1 in plant defense, specifically in response to herbivory and pathogen attack, and offer insights into the RERJ1-mediated regulation of metabolic pathways of specialized defense compounds, such as monoterpene linalool, in possible collaboration with OsMYC2—a well-known master regulator in jasmonate signaling. Abstract: In rice (Oryza sativa L.), the basic helix–loop–helix (bHLH) family transcription factor RERJ1 is induced under environmental stresses, such as wounding and drought, which are closely linked to jasmonate (JA) accumulation. Here, we investigated the biological function of RERJ1 in response to biotic stresses, such as herbivory and pathogen infection, using an RERJ1-defective mutant. Transcriptome analysis of the rerj1-Tos17 mutant revealed that RERJ1 regulated the expression of a typical family of conserved JA-responsive genes (e.g., terpene synthases, proteinase inhibitors, and jasmonate ZIM domain proteins). Upon exposure to armyworm attack, the rerj1-Tos17 mutant exhibited more severe damage than the wildtype, and significant weight gain of the larvae fed on the mutant was observed. Upon Xanthomonas oryzae infection, the rerj1-Tos17 mutant developed more severe symptoms than the wildtype. Among RERJ1-regulated terpene synthases, linalool synthase expression was markedly disrupted and linalool emission after wounding was significantly decreased in the rerj1-Tos17 mutant. RERJ1 appears to interact with OsMYC2—a master regulator of JA signaling—and many OsJAZ proteins, although no obvious epistatic interaction was detected between them at the transcriptional level. These results indicate that RERJ1 is involved in the transcriptional induction of JA-mediated stress-responsive genes via physical association with OsMYC2 and mediates defense against herbivory and bacterial infection through JA signaling.
KW - bHLH transcription factor
KW - Biotic stress
KW - Defense
KW - Jasmonate
KW - Oryza sativa
KW - Volatile
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UR - http://www.scopus.com/inward/citedby.url?scp=85114112803&partnerID=8YFLogxK
U2 - 10.1007/s11103-021-01186-0
DO - 10.1007/s11103-021-01186-0
M3 - Article
AN - SCOPUS:85114112803
JO - Plant Molecular Biology
JF - Plant Molecular Biology
SN - 0167-4412
ER -