The response of human T lymphocytes against alloantigens following Fas-mediated activation-induced cell death

T. Fujiwara, Hirofumi Fujita, S. Muranaka, K. Utsumi, S. Kusaka, K. Hamazaki

Research output: Contribution to journalArticle

Abstract

We examined the response of T lymphocytes activated with specific alloantigens following Fas-mediated apoptosis; using a mixed lymphocyte culture (MLC) system. Cells obtained from an MLC after 6 or 7 days of culture were incubated for are additional 24 hours in the presence or absence of the agonistic monoclonal antibody (MoAb), 7C11, or the antagonistic MoAb, ZB4. We assessed DNA fragmentation/specific cytotoxiy of the MoAb-treated cells. Cells harvested after 4 days of culture were sensitive to apoptosis induced by 7C11 with maximum DNA fragmentation observed on day 6. ZB4 slightly inhibited apoptosis of the cells compared with controls. The simultaneous addition of recombinant interleukin-2 (rIL-2) with the MoAbs significantly inhibited DNA fragmentation in control and ZB4-treated cells, but had little effect on the 7C11-treated cells. Control and ZB4-treated MLC cells showed cytotoxic activities against specific target cells, namely >10%. In contrast, the 7C11-treated cells showed

Original languageEnglish
Pages (from-to)46-48
Number of pages3
JournalTransplantation Proceedings
Volume37
Issue number1
DOIs
Publication statusPublished - Jan 2005
Externally publishedYes

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Isoantigens
Cell Death
T-Lymphocytes
DNA Fragmentation
Monoclonal Antibodies
Lymphocytes
Apoptosis
Interleukin-2
Cell Culture Techniques

ASJC Scopus subject areas

  • Surgery
  • Transplantation

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The response of human T lymphocytes against alloantigens following Fas-mediated activation-induced cell death. / Fujiwara, T.; Fujita, Hirofumi; Muranaka, S.; Utsumi, K.; Kusaka, S.; Hamazaki, K.

In: Transplantation Proceedings, Vol. 37, No. 1, 01.2005, p. 46-48.

Research output: Contribution to journalArticle

Fujiwara, T. ; Fujita, Hirofumi ; Muranaka, S. ; Utsumi, K. ; Kusaka, S. ; Hamazaki, K. / The response of human T lymphocytes against alloantigens following Fas-mediated activation-induced cell death. In: Transplantation Proceedings. 2005 ; Vol. 37, No. 1. pp. 46-48.
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