The Relationships between Development and Low Temperature Tolerance in Barley Near Isogenic Lines Differing for Flowering Behavior

Alfonso Cuesta-Marcos, Mariá Munõz-Amatriaín, Tanya Filichkin, Ildikó Karsai, Ben Trevaskis, Shozo Yasuda, Patrick Hayes, Kazuhiro Sato

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Flowering time, vernalization requirement, photoperiod sensitivity and low temperature tolerance are key traits in the Triticeae. We characterized a set of isogenic genetic stocks-representing single and pairwise substitutions of spring alleles at the VRN-H1, VRN-H2 and VRN-H3 loci in a winter barley background-at the structural, functional and phenotypic levels. High density mapping with reference to the barley genome sequence confirmed that in all cases target VRN alleles were present in the near isogenic lines (NILs) and allowed estimates of introgression size (at the genetic and physical levels) and gene content. Expression data corroborated the structural and phenotypic results. The latter confirmed that substitution of a spring allele at any of the VRN loci is sufficient to eliminate vernalization requirement. There was no significant change in low temperature tolerance with substitution of a spring allele at VRN-H2, but there were significant losses in cold tolerance with substitutions at VRN-H1 and VRN-H3. Reductions in cold tolerance are ascribed to an accelerated transition from the vegetative to reproductive state. The set of NILs will be a rich resource for understanding the genetics of vernalization, low temperature tolerance and other traits encoded/regulated by genes within the introgressed intervals.

Original languageEnglish
Pages (from-to)2312-2324
Number of pages13
JournalPlant and Cell Physiology
Volume56
Issue number12
DOIs
Publication statusPublished - Apr 9 2015

Fingerprint

isogenic lines
Hordeum
vernalization
Alleles
barley
flowering
alleles
Temperature
cold tolerance
temperature
winter barley
loci
Photoperiod
genetic resources
introgression
Genes
genes
photoperiod
Genome
genome

Keywords

  • Facultative growth habit
  • Hordeum vulgare L
  • Low temperature tolerance
  • Near isogenic lines
  • Vernalization

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

The Relationships between Development and Low Temperature Tolerance in Barley Near Isogenic Lines Differing for Flowering Behavior. / Cuesta-Marcos, Alfonso; Munõz-Amatriaín, Mariá; Filichkin, Tanya; Karsai, Ildikó; Trevaskis, Ben; Yasuda, Shozo; Hayes, Patrick; Sato, Kazuhiro.

In: Plant and Cell Physiology, Vol. 56, No. 12, 09.04.2015, p. 2312-2324.

Research output: Contribution to journalArticle

Cuesta-Marcos, A, Munõz-Amatriaín, M, Filichkin, T, Karsai, I, Trevaskis, B, Yasuda, S, Hayes, P & Sato, K 2015, 'The Relationships between Development and Low Temperature Tolerance in Barley Near Isogenic Lines Differing for Flowering Behavior', Plant and Cell Physiology, vol. 56, no. 12, pp. 2312-2324. https://doi.org/10.1093/pcp/pcv147
Cuesta-Marcos, Alfonso ; Munõz-Amatriaín, Mariá ; Filichkin, Tanya ; Karsai, Ildikó ; Trevaskis, Ben ; Yasuda, Shozo ; Hayes, Patrick ; Sato, Kazuhiro. / The Relationships between Development and Low Temperature Tolerance in Barley Near Isogenic Lines Differing for Flowering Behavior. In: Plant and Cell Physiology. 2015 ; Vol. 56, No. 12. pp. 2312-2324.
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