Abstract
Using Caco-2 cell monolayers and MTT assay, the relationship between cell viability (a) and transport-enhancement effect of 1,2-dicaproin (C6DG), monocaprin (C10MG), and capric acid sodium salt (C10FANa) was examined. Transport enhancement effect was assessed by apparent permeability (Papp) of penicillin V. There was a linear relationship between (Papp-aSa) and (1-a) values, where Sa was the apparent permeability for the viable cells. The apparent permeability for the damaged cells (Sd) was evaluated from the slope of the line. Each of the enhancer compounds gave a different Sd value 2.00 × 10-4, 0.82 × 10-4, and 0.10 × 10-4 cm/s for C6DG, C10MG, and C10FANa, respectively, but the value was independent of its concentration for C10MG and C10FANa. C6DG would be the safest enhancer among the three compounds because of its high Sd value at the low level of cell damage. Sd could be used as a criterion for estimating the safety of enhancers.
| Original language | English |
|---|---|
| Pages (from-to) | 83-86 |
| Number of pages | 4 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 62 |
| Issue number | 1 |
| Publication status | Published - Jan 1998 |
| Externally published | Yes |
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Keywords
- Caco-2
- Cell damage
- Medium chain fatty acid
- MTT
- Transport enhancer
ASJC Scopus subject areas
- Food Science
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biotechnology
- Chemistry (miscellaneous)
- Applied Microbiology and Biotechnology
Cite this
The Relationship between Transport-enhancement Effects and Cell Viability by Capric Acid Sodium Salt, Monocaprin, and Dicaproin. / Shima, Motohiro; Kimura, Yukitaka; Adachi, Shuji; Matsuno, Ryuichi.
In: Bioscience, Biotechnology and Biochemistry, Vol. 62, No. 1, 01.1998, p. 83-86.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Relationship between Transport-enhancement Effects and Cell Viability by Capric Acid Sodium Salt, Monocaprin, and Dicaproin
AU - Shima, Motohiro
AU - Kimura, Yukitaka
AU - Adachi, Shuji
AU - Matsuno, Ryuichi
PY - 1998/1
Y1 - 1998/1
N2 - Using Caco-2 cell monolayers and MTT assay, the relationship between cell viability (a) and transport-enhancement effect of 1,2-dicaproin (C6DG), monocaprin (C10MG), and capric acid sodium salt (C10FANa) was examined. Transport enhancement effect was assessed by apparent permeability (Papp) of penicillin V. There was a linear relationship between (Papp-aSa) and (1-a) values, where Sa was the apparent permeability for the viable cells. The apparent permeability for the damaged cells (Sd) was evaluated from the slope of the line. Each of the enhancer compounds gave a different Sd value 2.00 × 10-4, 0.82 × 10-4, and 0.10 × 10-4 cm/s for C6DG, C10MG, and C10FANa, respectively, but the value was independent of its concentration for C10MG and C10FANa. C6DG would be the safest enhancer among the three compounds because of its high Sd value at the low level of cell damage. Sd could be used as a criterion for estimating the safety of enhancers.
AB - Using Caco-2 cell monolayers and MTT assay, the relationship between cell viability (a) and transport-enhancement effect of 1,2-dicaproin (C6DG), monocaprin (C10MG), and capric acid sodium salt (C10FANa) was examined. Transport enhancement effect was assessed by apparent permeability (Papp) of penicillin V. There was a linear relationship between (Papp-aSa) and (1-a) values, where Sa was the apparent permeability for the viable cells. The apparent permeability for the damaged cells (Sd) was evaluated from the slope of the line. Each of the enhancer compounds gave a different Sd value 2.00 × 10-4, 0.82 × 10-4, and 0.10 × 10-4 cm/s for C6DG, C10MG, and C10FANa, respectively, but the value was independent of its concentration for C10MG and C10FANa. C6DG would be the safest enhancer among the three compounds because of its high Sd value at the low level of cell damage. Sd could be used as a criterion for estimating the safety of enhancers.
KW - Caco-2
KW - Cell damage
KW - Medium chain fatty acid
KW - MTT
KW - Transport enhancer
UR - http://www.scopus.com/inward/record.url?scp=0011254370&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0011254370&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0011254370
VL - 62
SP - 83
EP - 86
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 1
ER -