The Relationship between Transport-enhancement Effects and cell viability by capric acid sodium salt, monocaprin, and dicaproin

Motohiro Shima, Yukitaka Kimura, Shuji Adachi, Ryuichi Matsuno

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Abstract

Using Caco-2 cell monolayers and MTT assay, the relationship between cell viability (a) and transport-enhancement effect of 1,2-dicaproin (C6DG), monocaprin (C10MG), and capric acid sodium salt (C10FANa) was examined. Transport enhancement effect was assessed by apparent permeability (Papp) of penicillin V. There was a linear relationship between (Papp-aSa) and (1-a) values, where Sa was the apparent permeability for the viable cells. The apparent permeability for the damaged cells (Sd) was evaluated from the slope of the line. Each of the enhancer compounds gave a different Sd value 2.00×10-4, 0.82×10-4, and 0.10×10-4 cm/s for C6DG, C10MG, and C10FANa, respectively, but the value was independent of its concentration for C10MG and C10FANa. C6DG would be the safest enhancer among the three compounds because of its high Sd value at the low level of cell damage. Sd could be used as a criterion for estimating the safety of enhancers.

Original languageEnglish
Pages (from-to)83-86
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number1
DOIs
Publication statusPublished - Jan 1 1998
Externally publishedYes

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Keywords

  • Caco-2
  • Cell damage
  • MTT
  • Medium chain fatty acid
  • Transport enhancer

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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