The Relationship between Transport-enhancement Effects and Cell Viability by Capric Acid Sodium Salt, Monocaprin, and Dicaproin

Motohiro Shima, Yukitaka Kimura, Shuji Adachi, Ryuichi Matsuno

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Using Caco-2 cell monolayers and MTT assay, the relationship between cell viability (a) and transport-enhancement effect of 1,2-dicaproin (C6DG), monocaprin (C10MG), and capric acid sodium salt (C10FANa) was examined. Transport enhancement effect was assessed by apparent permeability (Papp) of penicillin V. There was a linear relationship between (Papp-aSa) and (1-a) values, where Sa was the apparent permeability for the viable cells. The apparent permeability for the damaged cells (Sd) was evaluated from the slope of the line. Each of the enhancer compounds gave a different Sd value 2.00 × 10-4, 0.82 × 10-4, and 0.10 × 10-4 cm/s for C6DG, C10MG, and C10FANa, respectively, but the value was independent of its concentration for C10MG and C10FANa. C6DG would be the safest enhancer among the three compounds because of its high Sd value at the low level of cell damage. Sd could be used as a criterion for estimating the safety of enhancers.

Original languageEnglish
Pages (from-to)83-86
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number1
Publication statusPublished - Jan 1998
Externally publishedYes

Fingerprint

decanoic acid
cell viability
Permeability
Cell Survival
Salts
Sodium
Cells
sodium
Penicillin V
salts
permeability
Monolayers
Assays
penicillin V
Caco-2 Cells
cells
Safety
rac-glycerol 1-monodecanoate
assays

Keywords

  • Caco-2
  • Cell damage
  • Medium chain fatty acid
  • MTT
  • Transport enhancer

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology

Cite this

The Relationship between Transport-enhancement Effects and Cell Viability by Capric Acid Sodium Salt, Monocaprin, and Dicaproin. / Shima, Motohiro; Kimura, Yukitaka; Adachi, Shuji; Matsuno, Ryuichi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 62, No. 1, 01.1998, p. 83-86.

Research output: Contribution to journalArticle

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