The relationship between pregnancy outcome and smooth endoplasmic reticulum clusters in MII human oocytes

Junko Otsuki, A. Okada, K. Morimoto, Y. Nagai, H. Kubo

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Background: During ICSI, we occasionally observe pronucleus sized translucent vacuoles. We investigated why these vacuoles occur and determined the effect on pregnancy outcome. Methods. Translucent vacuole-positive oocytes and the corresponding cohort were examined by transmission electron microscopy (TEM) and histochemical staining with Dil and ER-Tracker. Stimulation methods, hormonal levels, patients' condition and grade of transferred embryos were compared between vacuole-positive and vacuole-negative cycles. Results. By TEM, we confirmed that the vacuoles were tubular-type smooth endoplasmic reticulum clusters (sERCs). Numerous small sERCs were also observed in the oocytes from the same cohort. Veeck's grades of transferred embryos were higher in sERC-positive cycles and fertilization rate was similar to those of sERC-negative cycles. However, in sERC-positive cycles, significantly lower pregnancy and higher biochemical pregnancy rates were shown. Serum estradiol levels on the day of hCG administration were significantly higher in sERC-positive cycles. Conclusions: The presence of sERCs is associated with lower chances of successful pregnancy, even in sERC-negative oocytes from the same cohort that are transferred along with the sERC-positive oocytes. High estradiol levels could be one of the causes of sERC formation.

Original languageEnglish
Pages (from-to)1591-1597
Number of pages7
JournalHuman Reproduction
Volume19
Issue number7
DOIs
Publication statusPublished - Jul 2004
Externally publishedYes

Fingerprint

Smooth Endoplasmic Reticulum
Pregnancy Outcome
Oocytes
Vacuoles
Transmission Electron Microscopy
Estradiol
Embryonic Structures
Pregnancy
Intracytoplasmic Sperm Injections
Pregnancy Rate
Fertilization
Staining and Labeling

Keywords

  • Biochemical pregnancy
  • High serum estradiol levels
  • Idiopathic infertility
  • Oocyte maturation
  • Smooth endoplasmic reticulum cluster

ASJC Scopus subject areas

  • Reproductive Medicine
  • Rehabilitation
  • Obstetrics and Gynaecology

Cite this

The relationship between pregnancy outcome and smooth endoplasmic reticulum clusters in MII human oocytes. / Otsuki, Junko; Okada, A.; Morimoto, K.; Nagai, Y.; Kubo, H.

In: Human Reproduction, Vol. 19, No. 7, 07.2004, p. 1591-1597.

Research output: Contribution to journalArticle

Otsuki, Junko ; Okada, A. ; Morimoto, K. ; Nagai, Y. ; Kubo, H. / The relationship between pregnancy outcome and smooth endoplasmic reticulum clusters in MII human oocytes. In: Human Reproduction. 2004 ; Vol. 19, No. 7. pp. 1591-1597.
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