Abstract
Background: During ICSI, we occasionally observe pronucleus sized translucent vacuoles. We investigated why these vacuoles occur and determined the effect on pregnancy outcome. Methods. Translucent vacuole-positive oocytes and the corresponding cohort were examined by transmission electron microscopy (TEM) and histochemical staining with Dil and ER-Tracker. Stimulation methods, hormonal levels, patients' condition and grade of transferred embryos were compared between vacuole-positive and vacuole-negative cycles. Results. By TEM, we confirmed that the vacuoles were tubular-type smooth endoplasmic reticulum clusters (sERCs). Numerous small sERCs were also observed in the oocytes from the same cohort. Veeck's grades of transferred embryos were higher in sERC-positive cycles and fertilization rate was similar to those of sERC-negative cycles. However, in sERC-positive cycles, significantly lower pregnancy and higher biochemical pregnancy rates were shown. Serum estradiol levels on the day of hCG administration were significantly higher in sERC-positive cycles. Conclusions: The presence of sERCs is associated with lower chances of successful pregnancy, even in sERC-negative oocytes from the same cohort that are transferred along with the sERC-positive oocytes. High estradiol levels could be one of the causes of sERC formation.
| Original language | English |
|---|---|
| Pages (from-to) | 1591-1597 |
| Number of pages | 7 |
| Journal | Human Reproduction |
| Volume | 19 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 2004 |
| Externally published | Yes |
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Keywords
- Biochemical pregnancy
- High serum estradiol levels
- Idiopathic infertility
- Oocyte maturation
- Smooth endoplasmic reticulum cluster
ASJC Scopus subject areas
- Reproductive Medicine
- Rehabilitation
- Obstetrics and Gynaecology
Cite this
The relationship between pregnancy outcome and smooth endoplasmic reticulum clusters in MII human oocytes. / Otsuki, Junko; Okada, A.; Morimoto, K.; Nagai, Y.; Kubo, H.
In: Human Reproduction, Vol. 19, No. 7, 07.2004, p. 1591-1597.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The relationship between pregnancy outcome and smooth endoplasmic reticulum clusters in MII human oocytes
AU - Otsuki, Junko
AU - Okada, A.
AU - Morimoto, K.
AU - Nagai, Y.
AU - Kubo, H.
PY - 2004/7
Y1 - 2004/7
N2 - Background: During ICSI, we occasionally observe pronucleus sized translucent vacuoles. We investigated why these vacuoles occur and determined the effect on pregnancy outcome. Methods. Translucent vacuole-positive oocytes and the corresponding cohort were examined by transmission electron microscopy (TEM) and histochemical staining with Dil and ER-Tracker. Stimulation methods, hormonal levels, patients' condition and grade of transferred embryos were compared between vacuole-positive and vacuole-negative cycles. Results. By TEM, we confirmed that the vacuoles were tubular-type smooth endoplasmic reticulum clusters (sERCs). Numerous small sERCs were also observed in the oocytes from the same cohort. Veeck's grades of transferred embryos were higher in sERC-positive cycles and fertilization rate was similar to those of sERC-negative cycles. However, in sERC-positive cycles, significantly lower pregnancy and higher biochemical pregnancy rates were shown. Serum estradiol levels on the day of hCG administration were significantly higher in sERC-positive cycles. Conclusions: The presence of sERCs is associated with lower chances of successful pregnancy, even in sERC-negative oocytes from the same cohort that are transferred along with the sERC-positive oocytes. High estradiol levels could be one of the causes of sERC formation.
AB - Background: During ICSI, we occasionally observe pronucleus sized translucent vacuoles. We investigated why these vacuoles occur and determined the effect on pregnancy outcome. Methods. Translucent vacuole-positive oocytes and the corresponding cohort were examined by transmission electron microscopy (TEM) and histochemical staining with Dil and ER-Tracker. Stimulation methods, hormonal levels, patients' condition and grade of transferred embryos were compared between vacuole-positive and vacuole-negative cycles. Results. By TEM, we confirmed that the vacuoles were tubular-type smooth endoplasmic reticulum clusters (sERCs). Numerous small sERCs were also observed in the oocytes from the same cohort. Veeck's grades of transferred embryos were higher in sERC-positive cycles and fertilization rate was similar to those of sERC-negative cycles. However, in sERC-positive cycles, significantly lower pregnancy and higher biochemical pregnancy rates were shown. Serum estradiol levels on the day of hCG administration were significantly higher in sERC-positive cycles. Conclusions: The presence of sERCs is associated with lower chances of successful pregnancy, even in sERC-negative oocytes from the same cohort that are transferred along with the sERC-positive oocytes. High estradiol levels could be one of the causes of sERC formation.
KW - Biochemical pregnancy
KW - High serum estradiol levels
KW - Idiopathic infertility
KW - Oocyte maturation
KW - Smooth endoplasmic reticulum cluster
UR - http://www.scopus.com/inward/record.url?scp=3242879533&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3242879533&partnerID=8YFLogxK
U2 - 10.1093/humrep/deh258
DO - 10.1093/humrep/deh258
M3 - Article
VL - 19
SP - 1591
EP - 1597
JO - Human Reproduction
JF - Human Reproduction
SN - 0268-1161
IS - 7
ER -