The relationship between in vivo nasal drug clearance and in vitro nasal mucociliary clearance

Application to the prediction of nasal drug absorption

Daisuke Inoue, Akiko Tanaka, Shunsuke Kimura, Akiko Kiriyama, Hidemasa Katsumi, Akira Yamamoto, Ken ichi Ogawara, Toshikiro Kimura, Kazutaka Higaki, Reiko Yutani, Toshiyasu Sakane, Tomoyuki Furubayashi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Drug absorption after nasal application is dependent on drug clearance from the nasal cavity, which is determined by nasal mucociliary clearance (MC). We previously developed an in vitro method to evaluate MC via the translocation velocity of fluorescent microspheres (VFMS) applied to excised rat nasal mucosa. In the present study, the relationship between in vivo nasal MC and in vitro VFMS was examined to optimize our PK model for the prediction of nasal drug absorption. Appropriate inhibitors (propranolol and atropine) and enhancers (terbutaline and acetylcholine chloride) of MC were utilized to modify MC. In vivo clearance of drug from the nasal cavity was determined from the disappearance of fluorescent microspheres (FMS) from the nasal cavity following nasal application to rats. The first order elimination rate constant, kmc, was determined from the disappearance profiles of FMS. kmc was decreased to 35.8% by propranolol and 52.6% by atropine, but increased to 117% by terbutaline and 168% by acetylcholine chloride. A significant linear correlation was observed between kmc and VFMS (r2 = 0.9745, p < 0.001). These results indicate that in vivo kmc can be estimated from the in vitro parameter, VFMS. By introducing linear correlation into our PK model, nasal drug absorption may be precisely estimated, even with changes in MC.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalEuropean Journal of Pharmaceutical Sciences
Volume117
DOIs
Publication statusPublished - May 30 2018

Fingerprint

Mucociliary Clearance
Microspheres
Nose
Nasal Cavity
Pharmaceutical Preparations
Terbutaline
Atropine
Propranolol
Acetylcholine
Nasal Mucosa
Nasal Absorption
In Vitro Techniques

Keywords

  • Mucociliary clearance
  • Mucosal absorption
  • Nasal absorption
  • Nasal formulation
  • Nasal residence
  • Prediction system

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

The relationship between in vivo nasal drug clearance and in vitro nasal mucociliary clearance : Application to the prediction of nasal drug absorption. / Inoue, Daisuke; Tanaka, Akiko; Kimura, Shunsuke; Kiriyama, Akiko; Katsumi, Hidemasa; Yamamoto, Akira; Ogawara, Ken ichi; Kimura, Toshikiro; Higaki, Kazutaka; Yutani, Reiko; Sakane, Toshiyasu; Furubayashi, Tomoyuki.

In: European Journal of Pharmaceutical Sciences, Vol. 117, 30.05.2018, p. 21-26.

Research output: Contribution to journalArticle

Inoue, D, Tanaka, A, Kimura, S, Kiriyama, A, Katsumi, H, Yamamoto, A, Ogawara, KI, Kimura, T, Higaki, K, Yutani, R, Sakane, T & Furubayashi, T 2018, 'The relationship between in vivo nasal drug clearance and in vitro nasal mucociliary clearance: Application to the prediction of nasal drug absorption', European Journal of Pharmaceutical Sciences, vol. 117, pp. 21-26. https://doi.org/10.1016/j.ejps.2018.01.032
Inoue, Daisuke ; Tanaka, Akiko ; Kimura, Shunsuke ; Kiriyama, Akiko ; Katsumi, Hidemasa ; Yamamoto, Akira ; Ogawara, Ken ichi ; Kimura, Toshikiro ; Higaki, Kazutaka ; Yutani, Reiko ; Sakane, Toshiyasu ; Furubayashi, Tomoyuki. / The relationship between in vivo nasal drug clearance and in vitro nasal mucociliary clearance : Application to the prediction of nasal drug absorption. In: European Journal of Pharmaceutical Sciences. 2018 ; Vol. 117. pp. 21-26.
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