The regulatory effect of fermentable sugar levels on the production of leukotoxin by Actinobacillus actinomycetemcomitans

Katsunori Mizoguchi, Hiroyuki Ohta, Atsushi Miyagi, Hidemi Kurihara, Shogo Takashiba, Keijiro Kato, Yoji Murayama, Kazuhiro Fukui

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

The relationship between sugar availability and RTX (repeats in toxin) cytotoxin (leukotoxin) production in the periodontopathic bacterium, Actinobacillus actinomycetemcomitans, was investigated using a chemostat. A. actinomycetemcomitans 301-b produced significant amounts of leukotoxin in anaerobic fructose-limited chemostat cultures at a dilution rate of 0.15 h-1 and at pH 7.0. When the growth limitation was relieved by pulsing the cultures with 50 or 150 mM fructose (final concentrations), leukotoxin production immediately stopped and the amount of cellular leukotoxin decreased until the culture was returned to fructose-limited conditions. Leukotoxin synthesis was also repressed in the chemostat cultures by pulsing with glucose but not with the non-fermentable sugar analog, α-methyl-D-glucoside. Leukotoxin production was also repressed by fructose in chemostat cultures of ATCC 33384, which is generally recognized as a non-leukotoxin-producing or minimally leukotoxic strain.

Original languageEnglish
Pages (from-to)161-166
Number of pages6
JournalFEMS Microbiology Letters
Volume146
Issue number1
DOIs
Publication statusPublished - Jan 1 1997

Keywords

  • Actinobacillus actinomycetemcomitans
  • chemostat culture
  • leukotoxin
  • sugar repression

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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