The regulation of sulfur use by ferrous ion in thiobacillus ferrooxidans

Tsuyoshi Sugio, Toru Hirose, Aya Oto, Kenji Inagaki, Tatsuo Tano

Research output: Contribution to journalArticle

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Abstract

The use of elemental sulfur (S°) as an energy source by T. ferrooxidans AP19-3 was completely inhibited by above 108mM of Fe2+ added to S° (l%)-salts medium, in which about 0.6 mM Fe2+remained after 30 days cultivation without being oxidized. In contrast, the use of Fe2+ by this strain was not inhibited by S°. It was found that specific activities of hydrogen sulfide: ferric ion oxidoreductase (SFORase) and sulfite: ferric ion oxidoreductase, which are crucial in sulfur and sulfite oxidations of this strain, were completely inhibited by 20 mM and 1 mM Fe2+, suggesting that this may be a main cause for the cells not using sulfur as an energy source. The SFORase activity of the cells incubated with S°-Fe2+-salts medium decreased to 39% of that in control cells without Fe2+, suggesting that Fe2+ added to the medium inhibits a sulfite: ferric ion oxidoreductase, and as a results sulfite ions are accumulated in the cells and this damages the SFORase. Sodium sulfite completely inhibited cell growth on S°-salts medium at 0.1 m M, but not on iron-salts medium.

Original languageEnglish
Pages (from-to)2017-2022
Number of pages6
JournalAgricultural and Biological Chemistry
Volume54
Issue number8
DOIs
Publication statusPublished - 1990

Fingerprint

Acidithiobacillus ferrooxidans
Thiobacillus
Sulfur
sulfur
Salts
Ions
iron
ions
oxidoreductases
Sulfites
sulfites
Sulfides
Oxidoreductases
salts
Hydrogen Sulfide
Cell growth
sulfides
Iron
Cells
cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The regulation of sulfur use by ferrous ion in thiobacillus ferrooxidans. / Sugio, Tsuyoshi; Hirose, Toru; Oto, Aya; Inagaki, Kenji; Tano, Tatsuo.

In: Agricultural and Biological Chemistry, Vol. 54, No. 8, 1990, p. 2017-2022.

Research output: Contribution to journalArticle

Sugio, Tsuyoshi ; Hirose, Toru ; Oto, Aya ; Inagaki, Kenji ; Tano, Tatsuo. / The regulation of sulfur use by ferrous ion in thiobacillus ferrooxidans. In: Agricultural and Biological Chemistry. 1990 ; Vol. 54, No. 8. pp. 2017-2022.
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