The regulation of bone resorption in tooth formation and eruption processes in mouse alveolar crest devoid of cathepsin K

Masayo Okaji, Hideaki Sakai, Eiko Sakai, Mitsue Shibata, Fumio Hashimoto, Yasuhiro Kobayashi, Noriaki Yoshida, Kuniaki Okamoto, Kenji Yamamoto, Yuzo Kato

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Osteoclastic bone resorption has recently been implicated in the tooth formation and eruption in alveolar bone. Cathepsin K (CK) is a cysteine proteinase expressed predominantly in osteoclasts and is believed to play a critical role in degradation of bone matrix proteins. Here we present evidence that the alveolar bone resorption is essential for the tooth formation and that eruption proceeds normally in CK-deficient (CK-/-) mice. Radiographic and histological analyses revealed that the alveolar bone from these animals had no significant abnormalities during the tooth development between 5 and 28 days after birth. The tooth crown was normally erupted through the alveolar bone layer at 28 days after birth. The number of tartrate-resistant acid phosphatase-positive multinuclear cells in the alveolar bone around the tooth germ was apparently increased in 5-day-old CK-/- mice compared with age-matched littermates. More important, however, the immunohistochemical localization of matrix metalloproteinase-9 (MMP-9) was clearly increased in the CK-/- osteoclasts. In contrast, no significant difference in the immunoreactivity for cathepsin D was observed between the CK-/- osteoclasts and the wild-type ones. These results indicate that CK-/- osteoclasts are fully differentiated and are capable of degrading the organic phase of alveolar bone during the tooth formation and eruption, which may result from the compensatory action by MMP-9 increasingly expressed in the osteoclasts.

Original languageEnglish
Pages (from-to)285-294
Number of pages10
JournalJournal Pharmacological Sciences
Volume91
Issue number4
Publication statusPublished - Apr 1 2003
Externally publishedYes

Fingerprint

Cathepsin K
Tooth Eruption
Bone Resorption
Osteoclasts
Bone and Bones
Matrix Metalloproteinase 9
Tooth Abnormalities
Parturition
Tooth Crown
Tooth Germ
Alveolar Bone Loss
Alveolar Epithelial Cells
Cathepsin D
Bone Matrix
Cysteine Proteases

Keywords

  • Cathepsin K
  • Knockout mice
  • Matrix metalloproteinase-9
  • Osteoclast
  • Tooth development

ASJC Scopus subject areas

  • Pharmacology

Cite this

Okaji, M., Sakai, H., Sakai, E., Shibata, M., Hashimoto, F., Kobayashi, Y., ... Kato, Y. (2003). The regulation of bone resorption in tooth formation and eruption processes in mouse alveolar crest devoid of cathepsin K. Journal Pharmacological Sciences, 91(4), 285-294.

The regulation of bone resorption in tooth formation and eruption processes in mouse alveolar crest devoid of cathepsin K. / Okaji, Masayo; Sakai, Hideaki; Sakai, Eiko; Shibata, Mitsue; Hashimoto, Fumio; Kobayashi, Yasuhiro; Yoshida, Noriaki; Okamoto, Kuniaki; Yamamoto, Kenji; Kato, Yuzo.

In: Journal Pharmacological Sciences, Vol. 91, No. 4, 01.04.2003, p. 285-294.

Research output: Contribution to journalArticle

Okaji, M, Sakai, H, Sakai, E, Shibata, M, Hashimoto, F, Kobayashi, Y, Yoshida, N, Okamoto, K, Yamamoto, K & Kato, Y 2003, 'The regulation of bone resorption in tooth formation and eruption processes in mouse alveolar crest devoid of cathepsin K', Journal Pharmacological Sciences, vol. 91, no. 4, pp. 285-294.
Okaji, Masayo ; Sakai, Hideaki ; Sakai, Eiko ; Shibata, Mitsue ; Hashimoto, Fumio ; Kobayashi, Yasuhiro ; Yoshida, Noriaki ; Okamoto, Kuniaki ; Yamamoto, Kenji ; Kato, Yuzo. / The regulation of bone resorption in tooth formation and eruption processes in mouse alveolar crest devoid of cathepsin K. In: Journal Pharmacological Sciences. 2003 ; Vol. 91, No. 4. pp. 285-294.
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