The Rc and Rd genes are involved in proanthocyanidin synthesis in rice pericarp

Tsutomu Furukawa, Masahiko Maekawa, Tomoyuki Oki, Ikuo Suda, Shigeru Iida, Hiroaki Shimada, Itsuro Takamure, Koh Ichi Kadowaki

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

Different colors, such as purple, brown, red and white, occur in the pericarp of rice. Here, two genes affecting proanthocyanidin synthesis in red- and brown-colored rice were elucidated. Genetic segregation analysis suggested that the Rd and A loci are identical, and both encode dihydroflavonol-4- reductase (DFR). The introduction of the DFR gene into an Rcrd mutant resulted in red-colored rice, which was brown in the original mutant, demonstrating that the Rd locus encodes the DFR protein. Accumulation of proanthocyanidins was observed in the transformants by the introduction of the Rd gene into the rice Rcrd line. Protein blot analysis showed that the DFR gene was translated in seeds with alternative translation initiation. A search for the Rc gene, which encodes a transacting regulatory factor, was conducted using available DNA markers and the Rice Genome Automated Annotation System program. Three candidate genes were identified and cloned from a rice RcRd line and subsequently introduced into a rice rcrd line. Brown-colored seeds were obtained from transgenic plants by the introduction of a gene containing the basic helix-loop-helix (bHLH) motif, demonstrating that the Rc gene encodes a bHLH protein. Comparison of the Rc locus among rice accessions showed that a 14-bp deletion occurred only in the rc locus.

Original languageEnglish
Pages (from-to)91-102
Number of pages12
JournalPlant Journal
Volume49
Issue number1
DOIs
Publication statusPublished - Jan 1 2007

Keywords

  • Alternative translation initiation
  • Basic helix-loop-helix protein
  • Dihydroflavonol-4-reductase
  • Proanthocyanidins
  • Rice
  • Seed color

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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  • Cite this

    Furukawa, T., Maekawa, M., Oki, T., Suda, I., Iida, S., Shimada, H., Takamure, I., & Kadowaki, K. I. (2007). The Rc and Rd genes are involved in proanthocyanidin synthesis in rice pericarp. Plant Journal, 49(1), 91-102. https://doi.org/10.1111/j.1365-313X.2006.02958.x