The pva operon is located on the megaplasmid of Sphingopyxis sp. strain 113P3 and is constitutively expressed, although expression is enhanced by PVA

Xiaoping Hu, Rie Mamoto, Yoshio Fujioka, Akio Tani, Kazuhide Kimbara, Fusako Kawai

    Research output: Contribution to journalArticlepeer-review

    19 Citations (Scopus)

    Abstract

    The upstream and downstream regions of the tentative pva operon including genes encoding oxidized polyvinyl alcohol (PVA) hydrolase (oph), PVA dehydrogenase (pvaA) and cytochrome c (cytC) from Sphingopyxis sp. strain 113P3 were sequenced. The resultant 7.9 kb sequence contained orf1 in the upstream region and orf2 and orf3 in the downstream region. Reverse transcription- polymerase chain reaction (PCR) analyses revealed that the intergenic regions between orf1 and oph or between cytC and orf2 were expressed neither in PVA medium nor glucose medium, indicating that the pva operon consists of three genes. A transcription start site was determined by 5′-rapid amplification of cDNA ends to be 428 bp upstream of the start codon of the oph. The stop codon of cytC was followed by a sequence of inverted repeats that could function as a factor-independent transcription terminator. Strain 113P3 had one megaplasmid including the pva operon. Northern blot hybridization for the three genes revealed that mRNA size was approximately 3 to 4 kb and expression was elevated in PVA medium compared to glucose medium.

    Original languageEnglish
    Pages (from-to)685-693
    Number of pages9
    JournalApplied Microbiology and Biotechnology
    Volume78
    Issue number4
    DOIs
    Publication statusPublished - Mar 2008

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology

    Fingerprint

    Dive into the research topics of 'The pva operon is located on the megaplasmid of Sphingopyxis sp. strain 113P3 and is constitutively expressed, although expression is enhanced by PVA'. Together they form a unique fingerprint.

    Cite this