The proximal gastric corpus is the most responsive site of motilin-induced contractions in the stomach of the Asian house shrew

Amrita Dudani, Sayaka Aizawa, Gong Zhi, Toru Tanaka, Takamichi Jogahara, Ichiro Sakata, Takafumi Sakai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The migrating motor complex (MMC) is responsible for emptying the stomach during the interdigestive period, in preparation for the next meal. It is known that gastric phase III of MMC starts from the proximal stomach and propagates the contraction downwards. We hypothesized that a certain region of the stomach must be more responsive to motilin than others, and that motilin-induced strong gastric contractions propagate from that site. Stomachs of the Suncus or Asian house shrew, a small insectivorous mammal, were dissected and the fundus, proximal corpus, distal corpus, and antrum were examined to study the effect of motilin using an organ bath experiment. Motilin-induced contractions differed in different parts of the stomach. Only the proximal corpus induced gastric contraction even at motilin 10−10 M, and strong contraction was induced by motilin 10−9 M in all parts of the stomach. The GPR38 mRNA expression was also higher in the proximal corpus than in the other sections, and the lowest expression was observed in the antrum. GPR38 mRNA expression varied with low expression in the mucosal layer and high expression in the muscle layer. Additionally, motilin-induced contractions in each dissected part of the stomach were inhibited by tetrodotoxin and atropine pretreatment. These results suggest that motilin reactivity is not consistent throughout the stomach, and an area of the proximal corpus including the cardia is the most sensitive to motilin.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalJournal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
DOIs
Publication statusAccepted/In press - Apr 9 2016

Fingerprint

motilin
Shrews
Motilin
shrews
contraction
Stomach
stomach
Migrating Myoelectric Complexes
small mammal
Suncus
Messenger RNA
muscle
Mammals
tetrodotoxin
Tetrodotoxin
gastric emptying
food preparation
Cardia
atropine
Atropine

Keywords

  • Cardia
  • Corpus
  • Migrating motor complex
  • Motilin
  • Suncus murinus

ASJC Scopus subject areas

  • Physiology
  • Ecology, Evolution, Behavior and Systematics
  • Animal Science and Zoology
  • Biochemistry
  • Endocrinology

Cite this

The proximal gastric corpus is the most responsive site of motilin-induced contractions in the stomach of the Asian house shrew. / Dudani, Amrita; Aizawa, Sayaka; Zhi, Gong; Tanaka, Toru; Jogahara, Takamichi; Sakata, Ichiro; Sakai, Takafumi.

In: Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, 09.04.2016, p. 1-11.

Research output: Contribution to journalArticle

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