The protruding domain of the coat protein of Melon necrotic spot virus is involved in compatibility with and transmission by the fungal vector Olpidium bornovanus

Takehiro Ohki, Fusamichi Akita, Tomofumi Mochizuki, Ayami Kanda, Takahide Sasaya, Shinya Tsuda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The Chi and W strains of Melon necrotic spot virus (MNSV) are efficiently transmitted by isolates Y1 and NW1, respectively, of the fungal vector Olpidium bornovanus. Analysis of chimeric viruses constructed by switching the coat protein (CP) gene between the two strains unveiled the involvement of the CP in the attachment of MNSV to zoospores of a compatible isolate of O. bornovanus and in the fungal transmission of the virus. Furthermore, analysis of the chimeric virus based on the Chi strain with the protruding domain of the CP from strain W suggested the involvement of the domain in compatibility with zoospore. Comparison of the three-dimensional structures between the CP of the two MNSV strains showed that many of the differences in these amino acid residues are present on the surface of the virus particles, suggesting that these affects the recognition of fungal vectors by the virus.

Original languageEnglish
Pages (from-to)129-134
Number of pages6
JournalVirology
Volume402
Issue number1
DOIs
Publication statusPublished - Jun 1 2010
Externally publishedYes

Keywords

  • Coat protein
  • Fungal transmission
  • Melon necrotic spot virus
  • Olpidium bornovanus

ASJC Scopus subject areas

  • Virology

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