The protein encoded by NCgl1221 in Corynebacterium glutamicum functions as a mechanosensitive channel

Ken Ichi Hashimoto, Koji Nakamura, Teruo Kuroda, Isamu Yabe, Tsuyoshi Nakamatsu, Hisashi Kawasaki

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The function of the NCgl1221-encoded protein of Corynebacterium glutamicum was analyzed using Bacillus subtilis as host because a method for preparing the giant provacuole required for electrophysiological studies has been established. Expression of NCgl1221 in a strain deficient in mscL and ykuT, both of which encode mechanosensitive channels, resulted in an 8.9-fold higher cell survival rate upon osmotic downshock than the control. Electrophysiological investigation showed that the giant provacuole prepared from this strain, expressing NCgl1221, exhibited significantly higher pressuredependent conductance than the control. These findings show that the NCgl1221-encoded protein functions as a mechanosensitive channel.

Original languageEnglish
Pages (from-to)2546-2549
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number12
DOIs
Publication statusPublished - 2010

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Keywords

  • Corynebacterium glutamicum
  • Electrophysiological study
  • Glutamate overproduction
  • Mechanosensitive channel
  • Patch-clamp method

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

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