TY - JOUR
T1 - The promotional effect of IL-22 on mineralization activity of periodontal ligament cells
AU - Kato-Kogoe, Nahoko
AU - Nishioka, Toshihiro
AU - Kawabe, Mutsuki
AU - Kataoka, Fusa
AU - Yamanegi, Koji
AU - Yamada, Naoko
AU - Hata, Masaki
AU - Yamamoto, Tadashi
AU - Nakasho, Keiji
AU - Urade, Masahiro
AU - Terada, Nobuyuki
AU - Ohyama, Hideki
N1 - Funding Information:
This study was supported, in part, by a Grant-in- Aid for Scientific Research (C) (No. 20592443 , No. 20599020 , No. 23593076 and No. 23593077 ) from the Japan Society for the Promotion of Science, and Strategic Program Grant for Research Infrastructure Development in Private Institutes.
PY - 2012/7
Y1 - 2012/7
N2 - Objectives: Interleukin (IL)-22 acts on non-immune cells to induce anti-microbial responses, protection from tissue damage, and enhance cell regeneration. However, little is known about the involvement of IL-22 in periodontal biology. This study investigated the biological effects of IL-22 on periodontal ligament (PDL) cells as part of studies to assess the involvement of IL-22 in periodontal disease. Materials and methods: Gene expression levels of IL-22 and its receptors in PDL cells and gingival tissue samples were evaluated by real-time PCR. Proliferative responses and mineralized-matrix forming activities of PDL cells were examined in the presence and absence of IL-22. Results: In contrast to the expression of IL-22 receptors detected in PDL tissues and their cell lines, gingival tissues showed modest or no gene expressions of IL-22. The production of several cytokines including IL-11, IL-8 and CCL2 was upregulated by IL-22 treatment of PDL cells in a dose-dependent manner. IL-22 treatment had no effect on the proliferative response in PDL cells. Meanwhile, IL-22 precipitated mineralized nodule formation and induced gene expressions of RUNX2, MSX2 and osteocalcin in PDL cells, suggesting that IL-22 enhances the mineralized matrix-forming activities of PDL cells. Conclusion: IL-22 has the potential to promote mineralizing activity in PDL cells and to develop appropriate regenerative therapy.
AB - Objectives: Interleukin (IL)-22 acts on non-immune cells to induce anti-microbial responses, protection from tissue damage, and enhance cell regeneration. However, little is known about the involvement of IL-22 in periodontal biology. This study investigated the biological effects of IL-22 on periodontal ligament (PDL) cells as part of studies to assess the involvement of IL-22 in periodontal disease. Materials and methods: Gene expression levels of IL-22 and its receptors in PDL cells and gingival tissue samples were evaluated by real-time PCR. Proliferative responses and mineralized-matrix forming activities of PDL cells were examined in the presence and absence of IL-22. Results: In contrast to the expression of IL-22 receptors detected in PDL tissues and their cell lines, gingival tissues showed modest or no gene expressions of IL-22. The production of several cytokines including IL-11, IL-8 and CCL2 was upregulated by IL-22 treatment of PDL cells in a dose-dependent manner. IL-22 treatment had no effect on the proliferative response in PDL cells. Meanwhile, IL-22 precipitated mineralized nodule formation and induced gene expressions of RUNX2, MSX2 and osteocalcin in PDL cells, suggesting that IL-22 enhances the mineralized matrix-forming activities of PDL cells. Conclusion: IL-22 has the potential to promote mineralizing activity in PDL cells and to develop appropriate regenerative therapy.
KW - IL-22
KW - Mineralization activity
KW - Osteoblastic differentiation
KW - Periodontal disease
KW - Periodontal ligament cells
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U2 - 10.1016/j.cyto.2012.03.024
DO - 10.1016/j.cyto.2012.03.024
M3 - Article
C2 - 22537848
AN - SCOPUS:84861709210
SN - 1043-4666
VL - 59
SP - 41
EP - 48
JO - Cytokine
JF - Cytokine
IS - 1
ER -