The promotional effect of IL-22 on mineralization activity of periodontal ligament cells

Nahoko Kato-Kogoe, Toshihiro Nishioka, Mutsuki Kawabe, Fusa Kataoka, Koji Yamanegi, Naoko Yamada, Masaki Hata, Tadashi Yamamoto, Keiji Nakasho, Masahiro Urade, Nobuyuki Terada, Hideki Ohyama

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objectives: Interleukin (IL)-22 acts on non-immune cells to induce anti-microbial responses, protection from tissue damage, and enhance cell regeneration. However, little is known about the involvement of IL-22 in periodontal biology. This study investigated the biological effects of IL-22 on periodontal ligament (PDL) cells as part of studies to assess the involvement of IL-22 in periodontal disease. Materials and methods: Gene expression levels of IL-22 and its receptors in PDL cells and gingival tissue samples were evaluated by real-time PCR. Proliferative responses and mineralized-matrix forming activities of PDL cells were examined in the presence and absence of IL-22. Results: In contrast to the expression of IL-22 receptors detected in PDL tissues and their cell lines, gingival tissues showed modest or no gene expressions of IL-22. The production of several cytokines including IL-11, IL-8 and CCL2 was upregulated by IL-22 treatment of PDL cells in a dose-dependent manner. IL-22 treatment had no effect on the proliferative response in PDL cells. Meanwhile, IL-22 precipitated mineralized nodule formation and induced gene expressions of RUNX2, MSX2 and osteocalcin in PDL cells, suggesting that IL-22 enhances the mineralized matrix-forming activities of PDL cells. Conclusion: IL-22 has the potential to promote mineralizing activity in PDL cells and to develop appropriate regenerative therapy.

Original languageEnglish
Pages (from-to)41-48
Number of pages8
JournalCytokine
Volume59
Issue number1
DOIs
Publication statusPublished - Jul 2012

Fingerprint

Periodontal Ligament
Ligaments
Gene expression
Tissue
Gene Expression
interleukin-22
Interleukin-11
Osteocalcin
Periodontal Diseases
Interleukin-8
Real-Time Polymerase Chain Reaction
Regeneration
Cells
Cytokines

Keywords

  • IL-22
  • Mineralization activity
  • Osteoblastic differentiation
  • Periodontal disease
  • Periodontal ligament cells

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology
  • Biochemistry
  • Molecular Biology

Cite this

Kato-Kogoe, N., Nishioka, T., Kawabe, M., Kataoka, F., Yamanegi, K., Yamada, N., ... Ohyama, H. (2012). The promotional effect of IL-22 on mineralization activity of periodontal ligament cells. Cytokine, 59(1), 41-48. https://doi.org/10.1016/j.cyto.2012.03.024

The promotional effect of IL-22 on mineralization activity of periodontal ligament cells. / Kato-Kogoe, Nahoko; Nishioka, Toshihiro; Kawabe, Mutsuki; Kataoka, Fusa; Yamanegi, Koji; Yamada, Naoko; Hata, Masaki; Yamamoto, Tadashi; Nakasho, Keiji; Urade, Masahiro; Terada, Nobuyuki; Ohyama, Hideki.

In: Cytokine, Vol. 59, No. 1, 07.2012, p. 41-48.

Research output: Contribution to journalArticle

Kato-Kogoe, N, Nishioka, T, Kawabe, M, Kataoka, F, Yamanegi, K, Yamada, N, Hata, M, Yamamoto, T, Nakasho, K, Urade, M, Terada, N & Ohyama, H 2012, 'The promotional effect of IL-22 on mineralization activity of periodontal ligament cells', Cytokine, vol. 59, no. 1, pp. 41-48. https://doi.org/10.1016/j.cyto.2012.03.024
Kato-Kogoe N, Nishioka T, Kawabe M, Kataoka F, Yamanegi K, Yamada N et al. The promotional effect of IL-22 on mineralization activity of periodontal ligament cells. Cytokine. 2012 Jul;59(1):41-48. https://doi.org/10.1016/j.cyto.2012.03.024
Kato-Kogoe, Nahoko ; Nishioka, Toshihiro ; Kawabe, Mutsuki ; Kataoka, Fusa ; Yamanegi, Koji ; Yamada, Naoko ; Hata, Masaki ; Yamamoto, Tadashi ; Nakasho, Keiji ; Urade, Masahiro ; Terada, Nobuyuki ; Ohyama, Hideki. / The promotional effect of IL-22 on mineralization activity of periodontal ligament cells. In: Cytokine. 2012 ; Vol. 59, No. 1. pp. 41-48.
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AB - Objectives: Interleukin (IL)-22 acts on non-immune cells to induce anti-microbial responses, protection from tissue damage, and enhance cell regeneration. However, little is known about the involvement of IL-22 in periodontal biology. This study investigated the biological effects of IL-22 on periodontal ligament (PDL) cells as part of studies to assess the involvement of IL-22 in periodontal disease. Materials and methods: Gene expression levels of IL-22 and its receptors in PDL cells and gingival tissue samples were evaluated by real-time PCR. Proliferative responses and mineralized-matrix forming activities of PDL cells were examined in the presence and absence of IL-22. Results: In contrast to the expression of IL-22 receptors detected in PDL tissues and their cell lines, gingival tissues showed modest or no gene expressions of IL-22. The production of several cytokines including IL-11, IL-8 and CCL2 was upregulated by IL-22 treatment of PDL cells in a dose-dependent manner. IL-22 treatment had no effect on the proliferative response in PDL cells. Meanwhile, IL-22 precipitated mineralized nodule formation and induced gene expressions of RUNX2, MSX2 and osteocalcin in PDL cells, suggesting that IL-22 enhances the mineralized matrix-forming activities of PDL cells. Conclusion: IL-22 has the potential to promote mineralizing activity in PDL cells and to develop appropriate regenerative therapy.

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