The primary structure of the Cl--Translocating ATPase, b subunit of Acetabularia acetabulum, which belongs to the F-type ATpase family

Chie Moritani, Toshitaka Ohhashi, Hiromi Kadowaki, Mitsuo Tagaya, Toshio Fukui, Friedrich Lottspeich, Dieter Oesterhelt, Mikiko Ikeda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The genes possibly encoding the b subunit (50 kDa) of the Cl - translocating ATPase of Acetabularia acetabulum were cloned from total RNA and from poly(A)1 RNA and sequenced. The deduced amino acid sequence of the open reading frame consisted of 478 amino acids and showed high similarity to the β subunit of chloroplast F1-ATPase. Gene fragments encoding the putative β subunit of chloroplast F1- (273 bp) and mitochondrial F1- ATPases (332 bp) were also cloned from A. acetabulum and sequenced, respectively. The deduced amino acid sequence of the chloroplast F1-ATPase showed 92.5% identity to the primary structure of the b subunit of the Cl - translocating ATPase, while the nucleotide sequences were 79.9% identical. The deduced amino acid sequence of the latter was 77.3% identical to that of the b subunit of the Cl-translocating ATPase and the nucleotide sequences were 67.5% identical. By Northern analysis, these three β-like genes were demonstrated to be transcribed with different sizes of RNA species. A putative chloroplast F1-β fragment also hybridized with chloroplast DNA isolated from the organism.

Original languageEnglish
Pages (from-to)115-124
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume339
Issue number1
DOIs
Publication statusPublished - Mar 1 1997

Keywords

  • ATPase
  • Acetabularia acetabulum
  • Cl -translocation
  • F-type
  • b subunit
  • cloning

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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