The presequence of the precursor to the nucleus-encoded 30 kDa protein of photosystem II in Euglena gracilis Z includes two hydrophobic domains

Yasushi Shigemori, Junko Inagaki, Hitoshi Mori, Mikio Nishimura, Sumio Takahashi, Yasusi Yamamoto

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A cDNA clone for the extrinsic 30 kDa protein (OEC30) of photosystem II in Euglena gracilis Z was isolated and characterized. The open reading frame of the cDNA encoded a polypeptide of 338 amino acids, which consisted of a long presequence of 93 amino acids and a mature polypeptide of 245 amino acids. Two hydrophobic domains were identified in the presequence, in contrast to the presence of a single hydrophobic domain in the presequence of the corresponding proteins from higher plants. At the N- and C-terminal regions, respectively, of the presequence, a signal-peptide-like sequence and a thylakoid-transfer domain were identified. The presence of a long and unique presequence in the precursor to OEC30 is probably related to the complexity of the intracellular processes required for the synthesis and/or transport of the protein in Euglena.

Original languageEnglish
Pages (from-to)209-215
Number of pages7
JournalPlant Molecular Biology
Volume24
Issue number1
DOIs
Publication statusPublished - Jan 1994

Fingerprint

Euglena gracilis
Photosystem II Protein Complex
photosystem II
Amino Acids
amino acids
polypeptides
Complementary DNA
Euglena
Plant Proteins
Peptides
Thylakoids
Proteins
proteins
transport proteins
Protein Sorting Signals
signal peptide
thylakoids
Open Reading Frames
open reading frames
Carrier Proteins

Keywords

  • cDNA cloning
  • chloroplast
  • Euglena gracilis Z
  • oxygen-evolving complex
  • protein transport

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The presequence of the precursor to the nucleus-encoded 30 kDa protein of photosystem II in Euglena gracilis Z includes two hydrophobic domains. / Shigemori, Yasushi; Inagaki, Junko; Mori, Hitoshi; Nishimura, Mikio; Takahashi, Sumio; Yamamoto, Yasusi.

In: Plant Molecular Biology, Vol. 24, No. 1, 01.1994, p. 209-215.

Research output: Contribution to journalArticle

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AU - Yamamoto, Yasusi

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