The presence of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) during meiosis improves porcine 'oocyte competence' as determined by early embryonic development after in-vitro fertilization

Hiroaki Funahashi, Eric W. McIntush, Michael F. Smith, Billy N. Day

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The present studies were designed to determine the effect of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) during meiosis or after in vitro fertilization on early embryonic development of porcine embryos. The presence of purified ovine TIMP-1 in culture medium after in vitro fertilization did not affect the incidence of embryo cleavage or development to the blastocyst stage at 48 h or 6 days after insemination, respectively. In contrast, when various concentrations (1.25, 2.50 and 5.00 μg/ml) of purified ovine TIMP-1 were added to the culture medium during the second half (24 h) of oocyte maturation, normal cleavage rates (64 ± 3% at 1.25 μg/ml, 57 ± 5% at 2.50 μg/ml and 56 ± 1% at 5.00 μg/ml) were higher (p

Original languageEnglish
Pages (from-to)265-271
Number of pages7
JournalJournal of Reproduction and Development
Volume45
Issue number4
Publication statusPublished - 1999

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interstitial collagenase
in vitro fertilization
meiosis
oocytes
embryogenesis
swine
embryo (animal)
culture media
sheep
blastocyst
insemination
incidence
tissues

Keywords

  • In vitro maturation
  • Oocyte
  • Oocyte competence
  • Pig
  • TIMP-1

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

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title = "The presence of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) during meiosis improves porcine 'oocyte competence' as determined by early embryonic development after in-vitro fertilization",
abstract = "The present studies were designed to determine the effect of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) during meiosis or after in vitro fertilization on early embryonic development of porcine embryos. The presence of purified ovine TIMP-1 in culture medium after in vitro fertilization did not affect the incidence of embryo cleavage or development to the blastocyst stage at 48 h or 6 days after insemination, respectively. In contrast, when various concentrations (1.25, 2.50 and 5.00 μg/ml) of purified ovine TIMP-1 were added to the culture medium during the second half (24 h) of oocyte maturation, normal cleavage rates (64 ± 3{\%} at 1.25 μg/ml, 57 ± 5{\%} at 2.50 μg/ml and 56 ± 1{\%} at 5.00 μg/ml) were higher (p",
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AU - McIntush, Eric W.

AU - Smith, Michael F.

AU - Day, Billy N.

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