The possibility of reducing xenoantigen levels with a novel gal 3′-sulfotransferase (GP3ST)

Masaru Koma, Shuji Miyagawa, Koichi Honke, Rie Nakai, Shinichirou Miyoshi, Mitsunori Ohta, Hikaru Matsuda, Ryota Shirakura, Naoyuki Taniguchi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Glycoprotein-3-sulfotransferase (GP3ST) is a key enzyme in downregulating the expression of Galα1,3Galβ1,4GlcNAc-R (the α-Gal epitope), via enzymatic competition with an α1,3 galactosyltransferase (α1,3GT), such as α2,6 sialyltransferase (α2,6ST). In this study, we report the dominance of GP3ST over α1,3GT using transfected pig endothelial cell (PEC) lines. The introduction of the GP3ST gene into PEC suppresses its antigenicity with respect to normal human pooled serum (NHS), including the α-Gal epitope and the Hanganutziu-Deicher (H-D) antigen, and, in addition, reduces the susceptibility to NHS in complement-mediated cell lysis. Western and lectin blot analyses of the products of parental PEC and its transfectants indicated that proteins smaller than 66 kDa have a diminished reactivity with NHS and the IB4 lectin. The levels of the α-Gal epitope in neutral glycosphingolipids were also decreased in the GP3ST transfectants as detected in thin layer chromatography by immunostaining. These data indicate that GP3ST is very effective in reducing xenoepitope levels.

Original languageEnglish
Pages (from-to)517-522
Number of pages6
JournalJournal of biochemistry
Volume131
Issue number4
DOIs
Publication statusPublished - Apr 2002

Keywords

  • GP3ST
  • Glycoantigen
  • Pig endothelial cell
  • Xenotransplantation
  • α2,6ST

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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