The polymerization agent, 2-methyl-4'-(methylthio)-2- morpholinopropiophenone induces caspases-3/7 in human blood mononuclear cells in vitro

Yoichi Kawasaki, Kenta Yagi, Chiaki Tsuboi, Miwa Morizane, Yoshihisa Kitamura, Toshiaki Sendo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Our previous studies detected the presence of a photoinitiator 2-methyl-4'-(methylthio)-2-morpholinopropiophenone (MTMP) in an intravenous (i.v.) injection bag solution. Importantly, MTMP has demonstrated cytotoxicity for normal human peripheral blood (PB) mononuclear cells (MNC). Cell death pathways have two well-known modes, apoptosis and necrosis. But it has not been clear whether MTMP induced apoptosis or necrosis in normal human PB MNC. In the present in vitro study, we examined normal human PB MNC for the frequencies of apoptosis and necrosis and changes upon exposure to MTMP. We observed time-dependent changes in MNC viability with MTMP. We also assessed the activity of caspases-3/7. The results demonstrated that MTMP induced apoptosis in normal human PB MNC after 24 h. In addition, MTMP induced caspases-3/7 in a time-dependent manner. In conclusion, we suggest that MTMP induces apoptosis in a caspase-dependent pathway in vitro.

Original languageEnglish
Pages (from-to)1640-1645
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume36
Issue number10
DOIs
Publication statusPublished - 2013

Fingerprint

Caspase 7
Caspase 3
Polymerization
Blood Cells
Apoptosis
Necrosis
Caspases
Intravenous Injections
Cell Survival
Cell Death
2-methyl-4'-(methylthio)-2-morpholinopropiophenone
In Vitro Techniques

Keywords

  • Apoptosis
  • Caspase-3
  • Caspase-7
  • Cytotoxicity
  • Human mononuclear cell
  • Photoinitiator

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

The polymerization agent, 2-methyl-4'-(methylthio)-2- morpholinopropiophenone induces caspases-3/7 in human blood mononuclear cells in vitro. / Kawasaki, Yoichi; Yagi, Kenta; Tsuboi, Chiaki; Morizane, Miwa; Kitamura, Yoshihisa; Sendo, Toshiaki.

In: Biological and Pharmaceutical Bulletin, Vol. 36, No. 10, 2013, p. 1640-1645.

Research output: Contribution to journalArticle

@article{4f7656d024124f03aabac3e27edfcde9,
title = "The polymerization agent, 2-methyl-4'-(methylthio)-2- morpholinopropiophenone induces caspases-3/7 in human blood mononuclear cells in vitro",
abstract = "Our previous studies detected the presence of a photoinitiator 2-methyl-4'-(methylthio)-2-morpholinopropiophenone (MTMP) in an intravenous (i.v.) injection bag solution. Importantly, MTMP has demonstrated cytotoxicity for normal human peripheral blood (PB) mononuclear cells (MNC). Cell death pathways have two well-known modes, apoptosis and necrosis. But it has not been clear whether MTMP induced apoptosis or necrosis in normal human PB MNC. In the present in vitro study, we examined normal human PB MNC for the frequencies of apoptosis and necrosis and changes upon exposure to MTMP. We observed time-dependent changes in MNC viability with MTMP. We also assessed the activity of caspases-3/7. The results demonstrated that MTMP induced apoptosis in normal human PB MNC after 24 h. In addition, MTMP induced caspases-3/7 in a time-dependent manner. In conclusion, we suggest that MTMP induces apoptosis in a caspase-dependent pathway in vitro.",
keywords = "Apoptosis, Caspase-3, Caspase-7, Cytotoxicity, Human mononuclear cell, Photoinitiator",
author = "Yoichi Kawasaki and Kenta Yagi and Chiaki Tsuboi and Miwa Morizane and Yoshihisa Kitamura and Toshiaki Sendo",
year = "2013",
doi = "10.1248/bpb.b12-01032",
language = "English",
volume = "36",
pages = "1640--1645",
journal = "Biological and Pharmaceutical Bulletin",
issn = "0918-6158",
publisher = "Pharmaceutical Society of Japan",
number = "10",

}

TY - JOUR

T1 - The polymerization agent, 2-methyl-4'-(methylthio)-2- morpholinopropiophenone induces caspases-3/7 in human blood mononuclear cells in vitro

AU - Kawasaki, Yoichi

AU - Yagi, Kenta

AU - Tsuboi, Chiaki

AU - Morizane, Miwa

AU - Kitamura, Yoshihisa

AU - Sendo, Toshiaki

PY - 2013

Y1 - 2013

N2 - Our previous studies detected the presence of a photoinitiator 2-methyl-4'-(methylthio)-2-morpholinopropiophenone (MTMP) in an intravenous (i.v.) injection bag solution. Importantly, MTMP has demonstrated cytotoxicity for normal human peripheral blood (PB) mononuclear cells (MNC). Cell death pathways have two well-known modes, apoptosis and necrosis. But it has not been clear whether MTMP induced apoptosis or necrosis in normal human PB MNC. In the present in vitro study, we examined normal human PB MNC for the frequencies of apoptosis and necrosis and changes upon exposure to MTMP. We observed time-dependent changes in MNC viability with MTMP. We also assessed the activity of caspases-3/7. The results demonstrated that MTMP induced apoptosis in normal human PB MNC after 24 h. In addition, MTMP induced caspases-3/7 in a time-dependent manner. In conclusion, we suggest that MTMP induces apoptosis in a caspase-dependent pathway in vitro.

AB - Our previous studies detected the presence of a photoinitiator 2-methyl-4'-(methylthio)-2-morpholinopropiophenone (MTMP) in an intravenous (i.v.) injection bag solution. Importantly, MTMP has demonstrated cytotoxicity for normal human peripheral blood (PB) mononuclear cells (MNC). Cell death pathways have two well-known modes, apoptosis and necrosis. But it has not been clear whether MTMP induced apoptosis or necrosis in normal human PB MNC. In the present in vitro study, we examined normal human PB MNC for the frequencies of apoptosis and necrosis and changes upon exposure to MTMP. We observed time-dependent changes in MNC viability with MTMP. We also assessed the activity of caspases-3/7. The results demonstrated that MTMP induced apoptosis in normal human PB MNC after 24 h. In addition, MTMP induced caspases-3/7 in a time-dependent manner. In conclusion, we suggest that MTMP induces apoptosis in a caspase-dependent pathway in vitro.

KW - Apoptosis

KW - Caspase-3

KW - Caspase-7

KW - Cytotoxicity

KW - Human mononuclear cell

KW - Photoinitiator

UR - http://www.scopus.com/inward/record.url?scp=84887113225&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84887113225&partnerID=8YFLogxK

U2 - 10.1248/bpb.b12-01032

DO - 10.1248/bpb.b12-01032

M3 - Article

C2 - 24088257

AN - SCOPUS:84887113225

VL - 36

SP - 1640

EP - 1645

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

SN - 0918-6158

IS - 10

ER -