The polymerization agent, 2-methyl-4'-(methylthio)-2- morpholinopropiophenone induces caspases-3/7 in human blood mononuclear cells in vitro

Yoichi Kawasaki, Kenta Yagi, Chiaki Tsuboi, Miwa Morizane, Yoshihisa Kitamura, Toshiaki Sendo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Our previous studies detected the presence of a photoinitiator 2-methyl-4'-(methylthio)-2-morpholinopropiophenone (MTMP) in an intravenous (i.v.) injection bag solution. Importantly, MTMP has demonstrated cytotoxicity for normal human peripheral blood (PB) mononuclear cells (MNC). Cell death pathways have two well-known modes, apoptosis and necrosis. But it has not been clear whether MTMP induced apoptosis or necrosis in normal human PB MNC. In the present in vitro study, we examined normal human PB MNC for the frequencies of apoptosis and necrosis and changes upon exposure to MTMP. We observed time-dependent changes in MNC viability with MTMP. We also assessed the activity of caspases-3/7. The results demonstrated that MTMP induced apoptosis in normal human PB MNC after 24 h. In addition, MTMP induced caspases-3/7 in a time-dependent manner. In conclusion, we suggest that MTMP induces apoptosis in a caspase-dependent pathway in vitro.

Original languageEnglish
Pages (from-to)1640-1645
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume36
Issue number10
DOIs
Publication statusPublished - Nov 11 2013

Keywords

  • Apoptosis
  • Caspase-3
  • Caspase-7
  • Cytotoxicity
  • Human mononuclear cell
  • Photoinitiator

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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