Abstract
Plastids of higher plants are semi-autonomous cellular organelles that have their own genome and transcription-translation machinery. Examples of plastid functions are photosynthesis and biosynthesis of starch, amino acids, lipids and pigments. Plastid functions are encoded in ∼ 120 plastid genes and ∼ 3,000 nuclear genes. Although many embryo and seedling lethal nuclear genes are required for chloroplast biogenesis, until now deletion of plastid genes either had no phenotypic consequence (8 genes), or caused a mutant phenotype but did not affect viability (13 genes). Here we identify an essential plastid gene. By using the CRE-lox site-specific recombination system we have deleted clpP1 (caseinolytic protease P1), one of the three genes (clpP1, ycf1 and ycf2) whose disruption had previously only been possible in a fraction of the 1,000-10,000 plastid genome copies in a cell. Loss of the clpP1 gene product, the ClpP1 protease subunit, results in ablation of the shoot system of tobacco plants, suggesting that ClpP1-mediated protein degradation is essential for shoot development.
| Original language | English |
|---|---|
| Pages (from-to) | 86-89 |
| Number of pages | 4 |
| Journal | Nature |
| Volume | 425 |
| Issue number | 6953 |
| DOIs | |
| Publication status | Published - Sep 4 2003 |
| Externally published | Yes |
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The plastid clpP1 protease gene is essential for plant development. / Kuroda, Hiroshi; Maliga, Pal.
In: Nature, Vol. 425, No. 6953, 04.09.2003, p. 86-89.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The plastid clpP1 protease gene is essential for plant development
AU - Kuroda, Hiroshi
AU - Maliga, Pal
PY - 2003/9/4
Y1 - 2003/9/4
N2 - Plastids of higher plants are semi-autonomous cellular organelles that have their own genome and transcription-translation machinery. Examples of plastid functions are photosynthesis and biosynthesis of starch, amino acids, lipids and pigments. Plastid functions are encoded in ∼ 120 plastid genes and ∼ 3,000 nuclear genes. Although many embryo and seedling lethal nuclear genes are required for chloroplast biogenesis, until now deletion of plastid genes either had no phenotypic consequence (8 genes), or caused a mutant phenotype but did not affect viability (13 genes). Here we identify an essential plastid gene. By using the CRE-lox site-specific recombination system we have deleted clpP1 (caseinolytic protease P1), one of the three genes (clpP1, ycf1 and ycf2) whose disruption had previously only been possible in a fraction of the 1,000-10,000 plastid genome copies in a cell. Loss of the clpP1 gene product, the ClpP1 protease subunit, results in ablation of the shoot system of tobacco plants, suggesting that ClpP1-mediated protein degradation is essential for shoot development.
AB - Plastids of higher plants are semi-autonomous cellular organelles that have their own genome and transcription-translation machinery. Examples of plastid functions are photosynthesis and biosynthesis of starch, amino acids, lipids and pigments. Plastid functions are encoded in ∼ 120 plastid genes and ∼ 3,000 nuclear genes. Although many embryo and seedling lethal nuclear genes are required for chloroplast biogenesis, until now deletion of plastid genes either had no phenotypic consequence (8 genes), or caused a mutant phenotype but did not affect viability (13 genes). Here we identify an essential plastid gene. By using the CRE-lox site-specific recombination system we have deleted clpP1 (caseinolytic protease P1), one of the three genes (clpP1, ycf1 and ycf2) whose disruption had previously only been possible in a fraction of the 1,000-10,000 plastid genome copies in a cell. Loss of the clpP1 gene product, the ClpP1 protease subunit, results in ablation of the shoot system of tobacco plants, suggesting that ClpP1-mediated protein degradation is essential for shoot development.
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UR - http://www.scopus.com/inward/citedby.url?scp=0042823973&partnerID=8YFLogxK
U2 - 10.1038/nature01909
DO - 10.1038/nature01909
M3 - Article
C2 - 12955146
AN - SCOPUS:0042823973
VL - 425
SP - 86
EP - 89
JO - Nature
JF - Nature
SN - 0028-0836
IS - 6953
ER -