The pH-dependent conformational change of eukaryotic translation initiation factor 5: Insights into partner-binding manner

Yuxin Ye, Meirong Chen, Koji Kato, Min Yao

Research output: Contribution to journalArticle

Abstract

In the process of eukaryotic translation, the formation of preinitiation complex 43S, which consists of a 40S subunit, the eIF2–GTP–Met-tRNAiMet ternary complex, eIF3, eIF1, eIF1A, and eIF5, is essential for translational quality control. Of those factors, eIF5 promotes the hydrolysis of eIF2-bound GTP to release eIF2-GDP in the complex for the recycling of eIF2. eIF5 appears to bind to the β subunit of eIF2 (eIF2β) via an interaction between aromatic/acidic residue-rich regions (AA-boxes) in the C-terminal domain of eIF5 (eIF5CTD) and three lysine clusters (K-boxes) in the N-terminal domain of eIF2β (eIF2βNTD). However, the details of this interaction are unclear, due to the lack of a structure for the eIF5-eIF2β complex, and the unavailability of an intact structure of eIF5, in which the AA-boxes are always disordered, with high flexibility. In this study, we solved two crystal structures of eIF5CTD from Candida albicans, which for the first time showed the AA-box2 of eIF5 presenting as an ordered helical structure. The structures exhibited different arrangements of AA-box2 under different pH values, which may reflect the dynamic nature of the interactions of eIF5CTD, and eIF2βNTD in the preinitiation complex.

Original languageEnglish
Pages (from-to)186-191
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume519
Issue number1
DOIs
Publication statusPublished - Oct 29 2019
Externally publishedYes

Fingerprint

Eukaryotic Initiation Factor-5
Eukaryotic Initiation Factors
Candida
Recycling
Guanosine Triphosphate
Candida albicans
Quality Control
Lysine
Quality control
Hydrolysis
Crystal structure

Keywords

  • AA-box
  • eIF2β
  • eIF5
  • K-box
  • Structure

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The pH-dependent conformational change of eukaryotic translation initiation factor 5 : Insights into partner-binding manner. / Ye, Yuxin; Chen, Meirong; Kato, Koji; Yao, Min.

In: Biochemical and Biophysical Research Communications, Vol. 519, No. 1, 29.10.2019, p. 186-191.

Research output: Contribution to journalArticle

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