The perineuronal proteoglycan surface coat in the adult rat brain, with special reference to its reactions to Gömöri's ammoniacal silver

Ryutaro Endo, Shinichiro Murakami, Yu Masuda, Takehito Taguchi, Aiji Ohtsuka, Kazunori Nishizaki, Takuro Murakami

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The present study showed that many neurons in the adult rat brain possessed a perineuronal sulfated proteoglycan surface coat which reacted to cationic iron colloid and aldehyde fuchsin. This surface coat was stained supravitally with Ehrlich's methylene blue and doubly stained with Ehrlich's methylene blue and aldehyde fuchsin. The surface coat was also stained with Gömöri's ammoniacal silver and doubly stained with Gömöri's ammoniacal silver and cationic iron colloid. The surface coat was usually expressed together with a nerve cell surface glycoprotein net detectable with lectin Wisteria floribunda agglutinin. These findings indicate that the perineuronal proteoglycan surface coat is identical to Cajal's superficial reticulum and contains some collagenous elements. It was further demonstrated that collagenase digestion erased Gömöri's ammoniacal silver impregnation within the perineuronal proteoglycan surface coat.

Original languageEnglish
Pages (from-to)111-118
Number of pages8
JournalActa Medica Okayama
Volume54
Issue number3
Publication statusPublished - 2000

Fingerprint

Proteoglycans
Rosaniline Dyes
Rats
Brain
Methylene Blue
Colloids
Aldehydes
Iron
Neurons
Reticulum
Membrane Glycoproteins
Collagenases
Lectins
Digestion
Impregnation
ammoniacal silver

Keywords

  • Brain
  • Cell surface glycoproteins
  • Extracellular matrix
  • Perineuronal proteoglycans

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

The perineuronal proteoglycan surface coat in the adult rat brain, with special reference to its reactions to Gömöri's ammoniacal silver. / Endo, Ryutaro; Murakami, Shinichiro; Masuda, Yu; Taguchi, Takehito; Ohtsuka, Aiji; Nishizaki, Kazunori; Murakami, Takuro.

In: Acta Medica Okayama, Vol. 54, No. 3, 2000, p. 111-118.

Research output: Contribution to journalArticle

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AU - Nishizaki, Kazunori

AU - Murakami, Takuro

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