The pattern of joining (JH) gene usage in the human IgH chain is established predominantly at the B precursor cell stage

Robert Wasserman, Yoshinori Ito, Naomi Galili, Masao Yamada, Betty Anne Reichard, Sara Shane, Beverly Lange, Giovanni Rovera

Research output: Contribution to journalArticle

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Abstract

Preferential utilization of JH and D genes has been demonstrated in the rearranged IgH chain in human peripheral B cells. We report here that the same hierarchy of JH gene usage is observed in leukemic cells arrested in the B precursor stage of differentiation. Specifically, JH4 and JH6 accounted for 42.9% and 35.7%, respectively, of the JH gene usage in the leukemias compared with an expected frequency of 16.7% assuming unbiased gene usage. Within the D gene families, the DN1 gene appears to be overutilized in both populations, representing about 15% of the total gene usage compared with an expected frequency of 3.2%. Because 21 of the 36 leukemias contained only nonproductive IgH rearrangements, the preferential gene usage could not have arisen from pre-B cells that have undergone clonal selection after a productive rearrangement but before surface Ig expression. Nonproductive rearrangements exhibited the biased gene usage seen for productive rearrangements. These findings suggest that a recombination bias favoring certain segments may be the actual mechanism responsible for the apparent preferential utilization of JH and D genes.

Original languageEnglish
Pages (from-to)511-516
Number of pages6
JournalJournal of Immunology
Volume149
Issue number2
Publication statusPublished - Jul 15 1992

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B-Lymphoid Precursor Cells
Genes
Leukemia
Gene Rearrangement
Genetic Recombination
B-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Wasserman, R., Ito, Y., Galili, N., Yamada, M., Reichard, B. A., Shane, S., ... Rovera, G. (1992). The pattern of joining (JH) gene usage in the human IgH chain is established predominantly at the B precursor cell stage. Journal of Immunology, 149(2), 511-516.

The pattern of joining (JH) gene usage in the human IgH chain is established predominantly at the B precursor cell stage. / Wasserman, Robert; Ito, Yoshinori; Galili, Naomi; Yamada, Masao; Reichard, Betty Anne; Shane, Sara; Lange, Beverly; Rovera, Giovanni.

In: Journal of Immunology, Vol. 149, No. 2, 15.07.1992, p. 511-516.

Research output: Contribution to journalArticle

Wasserman, R, Ito, Y, Galili, N, Yamada, M, Reichard, BA, Shane, S, Lange, B & Rovera, G 1992, 'The pattern of joining (JH) gene usage in the human IgH chain is established predominantly at the B precursor cell stage', Journal of Immunology, vol. 149, no. 2, pp. 511-516.
Wasserman, Robert ; Ito, Yoshinori ; Galili, Naomi ; Yamada, Masao ; Reichard, Betty Anne ; Shane, Sara ; Lange, Beverly ; Rovera, Giovanni. / The pattern of joining (JH) gene usage in the human IgH chain is established predominantly at the B precursor cell stage. In: Journal of Immunology. 1992 ; Vol. 149, No. 2. pp. 511-516.
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