TY - JOUR
T1 - The origin, meiotic behavior, and transmission of a novel minichromosome in Arabidopsis thaliana
AU - Murata, Minoru
AU - Shibata, Fukashi
AU - Yokota, Etsuko
N1 - Funding Information:
Acknowledgements We thank Kazunari Kashihara for technical assistance, Paul B. Talbert for critical reading of the manuscript, and the Arabidopsis Biological Center at Ohio State University for providing seeds of Arabidopsis trisomic series and DNA clones used in this study. This work was supported by CREST, Japan Science and Technology, Japan.
PY - 2006/7
Y1 - 2006/7
N2 - A plant carrying a small extra chromosome was found in Landsberg erecta ecotype of Arabidopsis thaliana. Fluorescence in situ hybridization revealed that this minichromosome was derived from the short arm of chromosome 4. The size of this "mini4S" chromosome was estimated to be ∼7.5 Mb on the basis of previously reported data and the amount of the centromeric major satellite (180-bp family) present, which was determined to be about 1 Mb, or about one third of that in the normal chromosome 4. No pairing between mini4S and its original chromosome 4 was observed at pachytene and metaphase 1 stages. The transmission of mini4S through pollen was limited, but about 30% of selfed progeny carried the mini4S chromosomes. The transmission rates considerably increased when the mini4S chromosomes were transferred to plants with a Columbia background by successive backcrosses. This suggests that the stability of the minichromosomes is controlled genetically by factors that can vary between ecotypes.
AB - A plant carrying a small extra chromosome was found in Landsberg erecta ecotype of Arabidopsis thaliana. Fluorescence in situ hybridization revealed that this minichromosome was derived from the short arm of chromosome 4. The size of this "mini4S" chromosome was estimated to be ∼7.5 Mb on the basis of previously reported data and the amount of the centromeric major satellite (180-bp family) present, which was determined to be about 1 Mb, or about one third of that in the normal chromosome 4. No pairing between mini4S and its original chromosome 4 was observed at pachytene and metaphase 1 stages. The transmission of mini4S through pollen was limited, but about 30% of selfed progeny carried the mini4S chromosomes. The transmission rates considerably increased when the mini4S chromosomes were transferred to plants with a Columbia background by successive backcrosses. This suggests that the stability of the minichromosomes is controlled genetically by factors that can vary between ecotypes.
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U2 - 10.1007/s00412-005-0045-1
DO - 10.1007/s00412-005-0045-1
M3 - Article
C2 - 16607510
AN - SCOPUS:33745238406
VL - 115
SP - 311
EP - 319
JO - Chromosoma
JF - Chromosoma
SN - 0009-5915
IS - 4
ER -