The nucleotide sequence of the env gene from the human provirus ERV3 and isolation and characterization of an ERV3-specific cDNA

Maurice Cohen, Marilyn Powers, Catherine O'Connell, Nobuyuki Kato

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

The nucleotide sequence of the env gene of a previously described human provirus (ERV3) has been determined beginning near the 3′-end of the pol gene and continuing through the 3′-LTR. Analysis of the nucleotide sequence revealed the presence of a long open reading frame of 1944 nucleotides that is capable of encoding a polypeptide that has characteristics of other retroviral glycoproteins and transmembrane proteins. These include the presence of seven potential glycosylation sites, a typical glycoprotein-transmembrane protein cleavage sequence, and amino acid homologies to the glycoproteins and transmembrane proteins of other retroviruses. Further, we have isolated an ERV3-specific cDNA clone from a library prepared from liver RNA of a 20-week human fetus. DNA sequence analysis of this clone revealed that it is identical to the ERV3 genomic clone in the 1110 nucleotides that were sequenced.

Original languageEnglish
Pages (from-to)449-458
Number of pages10
JournalVirology
Volume147
Issue number2
DOIs
Publication statusPublished - 1985
Externally publishedYes

Fingerprint

env Genes
Proviruses
Glycoproteins
Complementary DNA
Clone Cells
Retroviridae Proteins
Nucleotides
pol Genes
Amino Acid Sequence Homology
DNA Sequence Analysis
Glycosylation
Open Reading Frames
Libraries
Proteins
Fetus
RNA
Peptides
Liver

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

The nucleotide sequence of the env gene from the human provirus ERV3 and isolation and characterization of an ERV3-specific cDNA. / Cohen, Maurice; Powers, Marilyn; O'Connell, Catherine; Kato, Nobuyuki.

In: Virology, Vol. 147, No. 2, 1985, p. 449-458.

Research output: Contribution to journalArticle

Cohen, Maurice ; Powers, Marilyn ; O'Connell, Catherine ; Kato, Nobuyuki. / The nucleotide sequence of the env gene from the human provirus ERV3 and isolation and characterization of an ERV3-specific cDNA. In: Virology. 1985 ; Vol. 147, No. 2. pp. 449-458.
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