TY - JOUR
T1 - The nucleotide sequence of the env gene from the human provirus ERV3 and isolation and characterization of an ERV3-specific cDNA
AU - Cohen, Maurice
AU - Powers, Marilyn
AU - O'Connell, Catherine
AU - Kato, Nobuyuki
N1 - Funding Information:
We thank Jeannie Clarke for preparing the manuscript. Researchw as sponsoredb y the National Cancer Institute, DHHS, under Contract NOl-CO-23909w ith Litton Bionetics, Inc. The contentso f this publication do not necessarily reflect the views or policies of the Department of Health and Human Services,n or does mention of trade names,c ommercialp roducts,o r organizations imply endorsementb y the U. S. government.
PY - 1985/12
Y1 - 1985/12
N2 - The nucleotide sequence of the env gene of a previously described human provirus (ERV3) has been determined beginning near the 3′-end of the pol gene and continuing through the 3′-LTR. Analysis of the nucleotide sequence revealed the presence of a long open reading frame of 1944 nucleotides that is capable of encoding a polypeptide that has characteristics of other retroviral glycoproteins and transmembrane proteins. These include the presence of seven potential glycosylation sites, a typical glycoprotein-transmembrane protein cleavage sequence, and amino acid homologies to the glycoproteins and transmembrane proteins of other retroviruses. Further, we have isolated an ERV3-specific cDNA clone from a library prepared from liver RNA of a 20-week human fetus. DNA sequence analysis of this clone revealed that it is identical to the ERV3 genomic clone in the 1110 nucleotides that were sequenced.
AB - The nucleotide sequence of the env gene of a previously described human provirus (ERV3) has been determined beginning near the 3′-end of the pol gene and continuing through the 3′-LTR. Analysis of the nucleotide sequence revealed the presence of a long open reading frame of 1944 nucleotides that is capable of encoding a polypeptide that has characteristics of other retroviral glycoproteins and transmembrane proteins. These include the presence of seven potential glycosylation sites, a typical glycoprotein-transmembrane protein cleavage sequence, and amino acid homologies to the glycoproteins and transmembrane proteins of other retroviruses. Further, we have isolated an ERV3-specific cDNA clone from a library prepared from liver RNA of a 20-week human fetus. DNA sequence analysis of this clone revealed that it is identical to the ERV3 genomic clone in the 1110 nucleotides that were sequenced.
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U2 - 10.1016/0042-6822(85)90147-3
DO - 10.1016/0042-6822(85)90147-3
M3 - Article
C2 - 3840930
AN - SCOPUS:0022344164
SN - 0042-6822
VL - 147
SP - 449
EP - 458
JO - Virology
JF - Virology
IS - 2
ER -