The non-mendelian green cotyledon gene in soybean encodes a small subunit of photosystem II

Kaori Kohzuma, Yutaka Sato, Hisashi Ito, Ayako Okuzaki, Mai Watanabe, Hideki Kobayashi, Michiharu Nakano, Hiroshi Yamatani, Yu Masuda, Yumi Nagashima, Hiroyuki Fukuoka, Tetsuya Yamada, Akira Kanazawa, Keisuke Kitamura, Yutaka Tabei, Masahiko Ikeuchi, Wataru Sakamoto, Ayumi Tanaka, Makoto Kusaba

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Chlorophyll degradation plays important roles in leaf senescence including regulation of degradation of chlorophyll-binding proteins. Although most genes encoding enzymes of the chlorophyll degradation pathway have been identified, the regulation of their activity has not been fully understood. Green cotyledon mutants in legume are stay-green mutants, in which chlorophyll degradation is impaired during leaf senescence and seed maturation. Among them, the soybean (Glycine max) green cotyledon gene cytG is unique because it is maternally inherited. To isolate cytG, we extensively sequenced the soybean chloroplast genome, and detected a 5-bp insertion causing a frame-shift in psbM, which encodes one of the small subunits of photosystem II. Mutant tobacco plants (Nicotiana tabacum) with a disrupted psbM generated using a chloroplast transformation technique had green senescent leaves, confirming that cytG encodes PsbM. The phenotype of cytG was very similar to that of mutant of chlorophyll b reductase catalyzing the first step of chlorophyll b degradation. In fact, chlorophyll b-degrading activity in dark-grown cytG and psbM-knockout seedlings was significantly lower than that of wild-type plants. Our results suggest that PsbM is a unique protein linking photosynthesis in presenescent leaves with chlorophyll degradation during leaf senescence and seed maturation. Additionally, we discuss the origin of cytG, which may have been selected during domestication of soybean.

Original languageEnglish
Pages (from-to)2138-2147
Number of pages10
JournalPlant Physiology
Volume173
Issue number4
DOIs
Publication statusPublished - Apr 1 2017

Fingerprint

Photosystem II Protein Complex
Cotyledon
Chlorophyll
Soybeans
photosystem II
cotyledons
soybeans
chlorophyll
degradation
Genes
Tobacco
genes
Chlorophyll Binding Proteins
mutants
Chloroplast Genome
seed maturation
leaves
Photosynthesis
Chloroplasts
Seedlings

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Kohzuma, K., Sato, Y., Ito, H., Okuzaki, A., Watanabe, M., Kobayashi, H., ... Kusaba, M. (2017). The non-mendelian green cotyledon gene in soybean encodes a small subunit of photosystem II. Plant Physiology, 173(4), 2138-2147. https://doi.org/10.1104/pp.16.01589

The non-mendelian green cotyledon gene in soybean encodes a small subunit of photosystem II. / Kohzuma, Kaori; Sato, Yutaka; Ito, Hisashi; Okuzaki, Ayako; Watanabe, Mai; Kobayashi, Hideki; Nakano, Michiharu; Yamatani, Hiroshi; Masuda, Yu; Nagashima, Yumi; Fukuoka, Hiroyuki; Yamada, Tetsuya; Kanazawa, Akira; Kitamura, Keisuke; Tabei, Yutaka; Ikeuchi, Masahiko; Sakamoto, Wataru; Tanaka, Ayumi; Kusaba, Makoto.

In: Plant Physiology, Vol. 173, No. 4, 01.04.2017, p. 2138-2147.

Research output: Contribution to journalArticle

Kohzuma, K, Sato, Y, Ito, H, Okuzaki, A, Watanabe, M, Kobayashi, H, Nakano, M, Yamatani, H, Masuda, Y, Nagashima, Y, Fukuoka, H, Yamada, T, Kanazawa, A, Kitamura, K, Tabei, Y, Ikeuchi, M, Sakamoto, W, Tanaka, A & Kusaba, M 2017, 'The non-mendelian green cotyledon gene in soybean encodes a small subunit of photosystem II', Plant Physiology, vol. 173, no. 4, pp. 2138-2147. https://doi.org/10.1104/pp.16.01589
Kohzuma K, Sato Y, Ito H, Okuzaki A, Watanabe M, Kobayashi H et al. The non-mendelian green cotyledon gene in soybean encodes a small subunit of photosystem II. Plant Physiology. 2017 Apr 1;173(4):2138-2147. https://doi.org/10.1104/pp.16.01589
Kohzuma, Kaori ; Sato, Yutaka ; Ito, Hisashi ; Okuzaki, Ayako ; Watanabe, Mai ; Kobayashi, Hideki ; Nakano, Michiharu ; Yamatani, Hiroshi ; Masuda, Yu ; Nagashima, Yumi ; Fukuoka, Hiroyuki ; Yamada, Tetsuya ; Kanazawa, Akira ; Kitamura, Keisuke ; Tabei, Yutaka ; Ikeuchi, Masahiko ; Sakamoto, Wataru ; Tanaka, Ayumi ; Kusaba, Makoto. / The non-mendelian green cotyledon gene in soybean encodes a small subunit of photosystem II. In: Plant Physiology. 2017 ; Vol. 173, No. 4. pp. 2138-2147.
@article{ba6347ce3f3a436f9e5daa5b64605e04,
title = "The non-mendelian green cotyledon gene in soybean encodes a small subunit of photosystem II",
abstract = "Chlorophyll degradation plays important roles in leaf senescence including regulation of degradation of chlorophyll-binding proteins. Although most genes encoding enzymes of the chlorophyll degradation pathway have been identified, the regulation of their activity has not been fully understood. Green cotyledon mutants in legume are stay-green mutants, in which chlorophyll degradation is impaired during leaf senescence and seed maturation. Among them, the soybean (Glycine max) green cotyledon gene cytG is unique because it is maternally inherited. To isolate cytG, we extensively sequenced the soybean chloroplast genome, and detected a 5-bp insertion causing a frame-shift in psbM, which encodes one of the small subunits of photosystem II. Mutant tobacco plants (Nicotiana tabacum) with a disrupted psbM generated using a chloroplast transformation technique had green senescent leaves, confirming that cytG encodes PsbM. The phenotype of cytG was very similar to that of mutant of chlorophyll b reductase catalyzing the first step of chlorophyll b degradation. In fact, chlorophyll b-degrading activity in dark-grown cytG and psbM-knockout seedlings was significantly lower than that of wild-type plants. Our results suggest that PsbM is a unique protein linking photosynthesis in presenescent leaves with chlorophyll degradation during leaf senescence and seed maturation. Additionally, we discuss the origin of cytG, which may have been selected during domestication of soybean.",
author = "Kaori Kohzuma and Yutaka Sato and Hisashi Ito and Ayako Okuzaki and Mai Watanabe and Hideki Kobayashi and Michiharu Nakano and Hiroshi Yamatani and Yu Masuda and Yumi Nagashima and Hiroyuki Fukuoka and Tetsuya Yamada and Akira Kanazawa and Keisuke Kitamura and Yutaka Tabei and Masahiko Ikeuchi and Wataru Sakamoto and Ayumi Tanaka and Makoto Kusaba",
year = "2017",
month = "4",
day = "1",
doi = "10.1104/pp.16.01589",
language = "English",
volume = "173",
pages = "2138--2147",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "4",

}

TY - JOUR

T1 - The non-mendelian green cotyledon gene in soybean encodes a small subunit of photosystem II

AU - Kohzuma, Kaori

AU - Sato, Yutaka

AU - Ito, Hisashi

AU - Okuzaki, Ayako

AU - Watanabe, Mai

AU - Kobayashi, Hideki

AU - Nakano, Michiharu

AU - Yamatani, Hiroshi

AU - Masuda, Yu

AU - Nagashima, Yumi

AU - Fukuoka, Hiroyuki

AU - Yamada, Tetsuya

AU - Kanazawa, Akira

AU - Kitamura, Keisuke

AU - Tabei, Yutaka

AU - Ikeuchi, Masahiko

AU - Sakamoto, Wataru

AU - Tanaka, Ayumi

AU - Kusaba, Makoto

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Chlorophyll degradation plays important roles in leaf senescence including regulation of degradation of chlorophyll-binding proteins. Although most genes encoding enzymes of the chlorophyll degradation pathway have been identified, the regulation of their activity has not been fully understood. Green cotyledon mutants in legume are stay-green mutants, in which chlorophyll degradation is impaired during leaf senescence and seed maturation. Among them, the soybean (Glycine max) green cotyledon gene cytG is unique because it is maternally inherited. To isolate cytG, we extensively sequenced the soybean chloroplast genome, and detected a 5-bp insertion causing a frame-shift in psbM, which encodes one of the small subunits of photosystem II. Mutant tobacco plants (Nicotiana tabacum) with a disrupted psbM generated using a chloroplast transformation technique had green senescent leaves, confirming that cytG encodes PsbM. The phenotype of cytG was very similar to that of mutant of chlorophyll b reductase catalyzing the first step of chlorophyll b degradation. In fact, chlorophyll b-degrading activity in dark-grown cytG and psbM-knockout seedlings was significantly lower than that of wild-type plants. Our results suggest that PsbM is a unique protein linking photosynthesis in presenescent leaves with chlorophyll degradation during leaf senescence and seed maturation. Additionally, we discuss the origin of cytG, which may have been selected during domestication of soybean.

AB - Chlorophyll degradation plays important roles in leaf senescence including regulation of degradation of chlorophyll-binding proteins. Although most genes encoding enzymes of the chlorophyll degradation pathway have been identified, the regulation of their activity has not been fully understood. Green cotyledon mutants in legume are stay-green mutants, in which chlorophyll degradation is impaired during leaf senescence and seed maturation. Among them, the soybean (Glycine max) green cotyledon gene cytG is unique because it is maternally inherited. To isolate cytG, we extensively sequenced the soybean chloroplast genome, and detected a 5-bp insertion causing a frame-shift in psbM, which encodes one of the small subunits of photosystem II. Mutant tobacco plants (Nicotiana tabacum) with a disrupted psbM generated using a chloroplast transformation technique had green senescent leaves, confirming that cytG encodes PsbM. The phenotype of cytG was very similar to that of mutant of chlorophyll b reductase catalyzing the first step of chlorophyll b degradation. In fact, chlorophyll b-degrading activity in dark-grown cytG and psbM-knockout seedlings was significantly lower than that of wild-type plants. Our results suggest that PsbM is a unique protein linking photosynthesis in presenescent leaves with chlorophyll degradation during leaf senescence and seed maturation. Additionally, we discuss the origin of cytG, which may have been selected during domestication of soybean.

UR - http://www.scopus.com/inward/record.url?scp=85016490587&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85016490587&partnerID=8YFLogxK

U2 - 10.1104/pp.16.01589

DO - 10.1104/pp.16.01589

M3 - Article

C2 - 28235890

AN - SCOPUS:85016490587

VL - 173

SP - 2138

EP - 2147

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 4

ER -