The NhaB Na+/H+ antiporter is essential for intracellular pH regulation under alkaline conditions in escherichia coli

Toshi Shimamoto, Kei Inaba, Paul Thelen, Tetsuya Ishikawa, Edward B. Goldberg, Masaaki Tsuda, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We isolated a mutant of Escherichia coli which was defective in an Na+/H+ antiporter and grew poorly under alkaline conditions [Ishikawa, T., Hama, EL, Tsuda, T., and Tsuchiya, T. (1987) J. BioL Chenu 262, 7443-7446]. Later, it was concluded that the defective Na+/H+ antiporter in the mutant was the NhaB system, and the nhaB gene was mapped to 25.6 min on the E. coli chromosome [Thelen, P., Tsuchiya, T., and Goldberg, E.B. (1991) J. Bacteriol. 173, 6553-6557]. We found that the NhaB-defective cells cannot grow in a high pH medium. Furthermore, intracellular pH in the mutant cells was almost the same as extracellular pH between 7.9 and 9.1, that is, intracellular pH was not regulated at this pH range. On the other hand, intracellular pH of the wild-type cells was maintained at about 7.6 when the extracellular pH was between 7.6 and 8.5. Thus, the NhaB Na+/H+ antiporter is essential for the regulation of intracellular pH under alkaline conditions in E. coli. Introduction of nhaA gene into the mutant cells increased Na+/H+ antiporter activity, but did not restore the defective growth and defective intracellular pH regulation under alkaline conditions.

Original languageEnglish
Pages (from-to)285-290
Number of pages6
JournalJournal of Biochemistry
Volume116
Issue number2
Publication statusPublished - Aug 1994

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Sodium-Hydrogen Antiporter
Mutant
Escherichia coli
Escherichia Coli
Cell
Genes
Chromosomes
Gene
Chromosome
Range of data

Keywords

  • Escherichia coli
  • NhaB antiporter
  • PH regulation
  • Sodium ion/proton antiporter

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Shimamoto, T., Inaba, K., Thelen, P., Ishikawa, T., Goldberg, E. B., Tsuda, M., & Tsuchiya, T. (1994). The NhaB Na+/H+ antiporter is essential for intracellular pH regulation under alkaline conditions in escherichia coli. Journal of Biochemistry, 116(2), 285-290.

The NhaB Na+/H+ antiporter is essential for intracellular pH regulation under alkaline conditions in escherichia coli. / Shimamoto, Toshi; Inaba, Kei; Thelen, Paul; Ishikawa, Tetsuya; Goldberg, Edward B.; Tsuda, Masaaki; Tsuchiya, Tomofusa.

In: Journal of Biochemistry, Vol. 116, No. 2, 08.1994, p. 285-290.

Research output: Contribution to journalArticle

Shimamoto, T, Inaba, K, Thelen, P, Ishikawa, T, Goldberg, EB, Tsuda, M & Tsuchiya, T 1994, 'The NhaB Na+/H+ antiporter is essential for intracellular pH regulation under alkaline conditions in escherichia coli', Journal of Biochemistry, vol. 116, no. 2, pp. 285-290.
Shimamoto, Toshi ; Inaba, Kei ; Thelen, Paul ; Ishikawa, Tetsuya ; Goldberg, Edward B. ; Tsuda, Masaaki ; Tsuchiya, Tomofusa. / The NhaB Na+/H+ antiporter is essential for intracellular pH regulation under alkaline conditions in escherichia coli. In: Journal of Biochemistry. 1994 ; Vol. 116, No. 2. pp. 285-290.
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