The neutral self-assembling peptide hydrogel SPG-178 as a topical hemostatic agent

Seiji Komatsu, Yusuke Nagai, Keiji Naruse, Yoshihiro Kimata

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Conventional self-assembling peptide hydrogels are effective as topical hemostatic agents. However, there is a possibility to harm living tissues due to their low pH. The aim of the present study was to demonstrate the efficacy of SPG-178, a neutral self-assembling peptide hydrogel, as a topical hemostatic agent. First, we measured the bleeding duration of incisions made on rat livers after application of SPG-178 (1.0% w/v), SPG-178 (1.5% w/v), RADA16 (1.0% w/v), and saline (n = 12/group). Second, we observed the bleeding surfaces by transmission electron microscopy immediately after hemostasis. Third, we measured the elastic and viscous responses (G′ and G″, respectively) of the hydrogels using a rheometer. Our results showed that bleeding duration was significantly shorter in the SPG-178 group than in the RADA16 group and that there were no significant differences in transmission electron microscopy findings between the groups. The greater the G′ value of a hydrogel, the shorter was the bleeding duration. We concluded that SPG-178 is more effective and has several advantages: it is non-biological, transparent, nonadherent, and neutral and can be sterilized by autoclaving.

Original languageEnglish
Article numbere102778
JournalPLoS One
Volume9
Issue number7
DOIs
Publication statusPublished - Jul 21 2014

Fingerprint

Hydrogels
Hydrogel
hydrocolloids
Hemostatics
hemorrhage
peptides
Hemorrhage
Transmission electron microscopy
Peptides
Rheometers
Transmission Electron Microscopy
Liver
duration
transmission electron microscopy
Rats
Tissue
hemostasis
autoclaving
Hemostasis
liver

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

The neutral self-assembling peptide hydrogel SPG-178 as a topical hemostatic agent. / Komatsu, Seiji; Nagai, Yusuke; Naruse, Keiji; Kimata, Yoshihiro.

In: PLoS One, Vol. 9, No. 7, e102778, 21.07.2014.

Research output: Contribution to journalArticle

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