TY - JOUR
T1 - The neutral self-assembling peptide hydrogel SPG-178 as a topical hemostatic agent
AU - Komatsu, Seiji
AU - Nagai, Yusuke
AU - Naruse, Keiji
AU - Kimata, Yoshihiro
N1 - Funding Information:
The authors have read the journal's policy and have the following conflicts: The authors wish to draw the attention of the editor to the following facts that may be considered as potential conflicts of interest and to significant financial contributions to this work. SPG-178 is a commercially available regent under the name of PanaceaGelTM (Menicon Co., Ltd., Nagoya, Japan). The Okayama Foundation also provided financial support for this study for Science and Technology (to Yoshihiro Kimata). Menicon Co., Ltd. is offering its products at subsidized prices to the Okayama Foundation for Science and Technology. Besides, Yusuke Nagai is an employee of Menicon Co., Ltd. and Keiji Naruse is a research/adviser of Menicon Co., Ltd. This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials.
PY - 2014/7/21
Y1 - 2014/7/21
N2 - Conventional self-assembling peptide hydrogels are effective as topical hemostatic agents. However, there is a possibility to harm living tissues due to their low pH. The aim of the present study was to demonstrate the efficacy of SPG-178, a neutral self-assembling peptide hydrogel, as a topical hemostatic agent. First, we measured the bleeding duration of incisions made on rat livers after application of SPG-178 (1.0% w/v), SPG-178 (1.5% w/v), RADA16 (1.0% w/v), and saline (n = 12/group). Second, we observed the bleeding surfaces by transmission electron microscopy immediately after hemostasis. Third, we measured the elastic and viscous responses (G′ and G″, respectively) of the hydrogels using a rheometer. Our results showed that bleeding duration was significantly shorter in the SPG-178 group than in the RADA16 group and that there were no significant differences in transmission electron microscopy findings between the groups. The greater the G′ value of a hydrogel, the shorter was the bleeding duration. We concluded that SPG-178 is more effective and has several advantages: it is non-biological, transparent, nonadherent, and neutral and can be sterilized by autoclaving.
AB - Conventional self-assembling peptide hydrogels are effective as topical hemostatic agents. However, there is a possibility to harm living tissues due to their low pH. The aim of the present study was to demonstrate the efficacy of SPG-178, a neutral self-assembling peptide hydrogel, as a topical hemostatic agent. First, we measured the bleeding duration of incisions made on rat livers after application of SPG-178 (1.0% w/v), SPG-178 (1.5% w/v), RADA16 (1.0% w/v), and saline (n = 12/group). Second, we observed the bleeding surfaces by transmission electron microscopy immediately after hemostasis. Third, we measured the elastic and viscous responses (G′ and G″, respectively) of the hydrogels using a rheometer. Our results showed that bleeding duration was significantly shorter in the SPG-178 group than in the RADA16 group and that there were no significant differences in transmission electron microscopy findings between the groups. The greater the G′ value of a hydrogel, the shorter was the bleeding duration. We concluded that SPG-178 is more effective and has several advantages: it is non-biological, transparent, nonadherent, and neutral and can be sterilized by autoclaving.
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U2 - 10.1371/journal.pone.0102778
DO - 10.1371/journal.pone.0102778
M3 - Article
C2 - 25047639
AN - SCOPUS:84904574505
VL - 9
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 7
M1 - e102778
ER -