The NB-LRR proteins RGA4 and RGA5 interact functionally and physically to confer disease resistance

Stella Césari, Hiroyuki Kanzaki, Tadashi Fujiwara, Maud Bernoux, Véronique Chalvon, Yoji Kawano, Ko Shimamoto, Peter Dodds, Ryohei Terauchi, Thomas Kroj

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

Plant resistance proteins of the class of nucleotide-binding and leucine-rich repeat domain proteins (NB-LRRs) are immune sensors which recognize pathogen-derived molecules termed avirulence (AVR) proteins. We show that RGA4 and RGA5, two NB-LRRs from rice, interact functionally and physically to mediate resistance to the fungal pathogen Magnaporthe oryzae and accomplish different functions in AVR recognition. RGA4 triggers an AVR-independent cell death that is repressed in the presence of RGA5 in both rice protoplasts and Nicotiana benthamiana. Upon recognition of the pathogen effector AVR-Pia by direct binding to RGA5, repression is relieved and cell death occurs. RGA4 and RGA5 form homo- and hetero-complexes and interact through their coiled-coil domains. Localization studies in rice protoplast suggest that RGA4 and RGA5 localize to the cytosol. Upon recognition of AVR-Pia, neither RGA4 nor RGA5 is re-localized to the nucleus. These results establish a model for the interaction of hetero-pairs of NB-LRRs in plants: RGA4 mediates cell death activation, while RGA5 acts as a repressor of RGA4 and as an AVR receptor. Synopsis Plant microbial resistance is mediated by a pair of interacting immune sensors, RGA4 and RGA5. RGA4 mediates cell death but is repressed by RGA5. The repressor is neutralized by binding of pathogen-derived proteins to the dimer. Rice NB-LRR pair RGA4 and RGA5 interact through their CC domains and form homo- and hetero-complexes. RGA4 triggers effector-independent resistance responses that are repressed by RGA5. Recognition and physical binding of the fungal effector protein AVR-Pia by RGA5 relieves repression and activates RGA4-dependent resistance signaling. Plant microbial resistance is mediated by a pair of interacting immune sensors, RGA4 and RGA5. RGA4 mediates cell death but is repressed by RGA5. The repressor is neutralized by binding of pathogen-derived proteins to the dimer.

Original languageEnglish
Pages (from-to)1941-1959
Number of pages19
JournalEMBO Journal
Volume33
Issue number17
DOIs
Publication statusPublished - Sep 1 2014

Keywords

  • Magnaporthe oryzae
  • pathogen recognition
  • plant immunity
  • resistance protein
  • rice

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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  • Cite this

    Césari, S., Kanzaki, H., Fujiwara, T., Bernoux, M., Chalvon, V., Kawano, Y., Shimamoto, K., Dodds, P., Terauchi, R., & Kroj, T. (2014). The NB-LRR proteins RGA4 and RGA5 interact functionally and physically to confer disease resistance. EMBO Journal, 33(17), 1941-1959. https://doi.org/10.15252/embj.201487923