The N-terminal region of hepatitis C virus-encoded NS5A is important for NS4A-dependent phosphorylation

Shin Ichi Asabe, Yasunori Tanji, Shinya Satoh, Takashi Kaneko, Koichi Kimura, Kunitada Shimotohno

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

We previously showed that two proteins, a 56-kDa protein (p56) and a 58- kDa protein (p58), are produced from the hepatitis C virus (HCV) nonstructural 5A region (NSSA) and that the production of p58 is enhanced by the presence of NS4A (T. Kaneko, Y. Tanji, S. Satoh, M. Hijikata, S. Asahe, K. Kimura, and K. Shimotohno, Biochem. Biophys. Res. Commun. 205:320-326, 1994). Both proteins have phosphorylated serine residues, some of which are located in the C-terminal region. In p58, phosphorylation of serine residues in the central region of HCV NS5A is important for production of p58 in an NS4A-dependent manner. To clarify the mechanism of NS5A phosphorylation, in particular phosphorylation in the central region, phosphorylation of deleted and mutated forms of NS5A was analyzed using a transient protein production system in cultured cells in the presence or absence of NS4A. Association of the NS5A region from amino acids 2135 to 2139 with NS4A was important for NS4A-dependent phosphorylation of NS5A.

Original languageEnglish
Pages (from-to)790-796
Number of pages7
JournalJournal of Virology
Volume71
Issue number1
Publication statusPublished - 1997
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Asabe, S. I., Tanji, Y., Satoh, S., Kaneko, T., Kimura, K., & Shimotohno, K. (1997). The N-terminal region of hepatitis C virus-encoded NS5A is important for NS4A-dependent phosphorylation. Journal of Virology, 71(1), 790-796.