The minimal tRNA: Unique structure of Ascaris suum mitochondrial tRNAUCUSer having a short T arm and lacking the entire D arm

Takashi Ohtsuki; Gota Kawai; Kimitsuna Watanabe

doi:10.1016/S0014-5793(02)02328-1

The minimal tRNA: Unique structure of Ascaris suum mitochondrial tRNA_UCU^Ser having a short T arm and lacking the entire D arm

Takashi Ohtsuki, Gota Kawai, Kimitsuna Watanabe

Research output: Contribution to journal › Article › peer-review

63 Citations (Scopus)

Abstract

The tertiary structure of Ascaris suum mitochondrial tRNA_UCU^Ser was examined by nuclear magnetic resonance analysis using its transcript, since tRNA_UCU^Ser, lacking the D arm and possessing a truncated T arm, is the shortest of all the known tRNAs. Most basepairs in the proposed secondary structure of tRNA_UCU^Ser were shown to exist, but the connector region comprising the truncated D loop and the extra loop was flexible. This flexibility, would enable adjustment of the mutual distance between the 3′-terminus and the anticodon consistent with that of usual tRNAs. Thus, tRNA^SerUCU appears to function in a similar way to that of usual tRNAs in the ribosome.

Original language	English
Pages (from-to)	37-43
Number of pages	7
Journal	FEBS Letters
Volume	514
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(02)02328-1
Publication status	Published - Mar 6 2002
Externally published	Yes

Keywords

Ascaris suum
Mitochondrion
Nematode
Nuclear magnetic resonance
Stable isotope
Unusual tRNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/S0014-5793(02)02328-1

Cite this

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title = "The minimal tRNA: Unique structure of Ascaris suum mitochondrial tRNAUCUSer having a short T arm and lacking the entire D arm",

abstract = "The tertiary structure of Ascaris suum mitochondrial tRNAUCUSer was examined by nuclear magnetic resonance analysis using its transcript, since tRNAUCUSer, lacking the D arm and possessing a truncated T arm, is the shortest of all the known tRNAs. Most basepairs in the proposed secondary structure of tRNAUCUSer were shown to exist, but the connector region comprising the truncated D loop and the extra loop was flexible. This flexibility, would enable adjustment of the mutual distance between the 3′-terminus and the anticodon consistent with that of usual tRNAs. Thus, tRNASerUCU appears to function in a similar way to that of usual tRNAs in the ribosome.",

keywords = "Ascaris suum, Mitochondrion, Nematode, Nuclear magnetic resonance, Stable isotope, Unusual tRNA",

author = "Takashi Ohtsuki and Gota Kawai and Kimitsuna Watanabe",

note = "Funding Information: We would like to thank Dr. Y. Watanabe for discussions and the material, Dr. K. Kita (University of Tokyo, Japan) for the A. suum mitochondria and Dr. C. Takemoto for E. coli tRNA Phe . This work was supported by Grants-in-Aid for Scientific Research on Priority Areas (No. 04272102 and No. 04272103) from the Ministry of Education, Science, Sports and Culture of Japan, JSPS (to T.O.) and by the Human Frontier Science Program Organization.",

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AU - Ohtsuki, Takashi

AU - Kawai, Gota

AU - Watanabe, Kimitsuna

N1 - Funding Information: We would like to thank Dr. Y. Watanabe for discussions and the material, Dr. K. Kita (University of Tokyo, Japan) for the A. suum mitochondria and Dr. C. Takemoto for E. coli tRNA Phe . This work was supported by Grants-in-Aid for Scientific Research on Priority Areas (No. 04272102 and No. 04272103) from the Ministry of Education, Science, Sports and Culture of Japan, JSPS (to T.O.) and by the Human Frontier Science Program Organization.

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N2 - The tertiary structure of Ascaris suum mitochondrial tRNAUCUSer was examined by nuclear magnetic resonance analysis using its transcript, since tRNAUCUSer, lacking the D arm and possessing a truncated T arm, is the shortest of all the known tRNAs. Most basepairs in the proposed secondary structure of tRNAUCUSer were shown to exist, but the connector region comprising the truncated D loop and the extra loop was flexible. This flexibility, would enable adjustment of the mutual distance between the 3′-terminus and the anticodon consistent with that of usual tRNAs. Thus, tRNASerUCU appears to function in a similar way to that of usual tRNAs in the ribosome.

AB - The tertiary structure of Ascaris suum mitochondrial tRNAUCUSer was examined by nuclear magnetic resonance analysis using its transcript, since tRNAUCUSer, lacking the D arm and possessing a truncated T arm, is the shortest of all the known tRNAs. Most basepairs in the proposed secondary structure of tRNAUCUSer were shown to exist, but the connector region comprising the truncated D loop and the extra loop was flexible. This flexibility, would enable adjustment of the mutual distance between the 3′-terminus and the anticodon consistent with that of usual tRNAs. Thus, tRNASerUCU appears to function in a similar way to that of usual tRNAs in the ribosome.

KW - Ascaris suum

KW - Mitochondrion

KW - Nematode

KW - Nuclear magnetic resonance

KW - Stable isotope

KW - Unusual tRNA

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