The minimal tRNA: Unique structure of Ascaris suum mitochondrial tRNAUCUSer having a short T arm and lacking the entire D arm

Takashi Ohtsuki, Gota Kawai, Kimitsuna Watanabe

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The tertiary structure of Ascaris suum mitochondrial tRNAUCUSer was examined by nuclear magnetic resonance analysis using its transcript, since tRNAUCUSer, lacking the D arm and possessing a truncated T arm, is the shortest of all the known tRNAs. Most basepairs in the proposed secondary structure of tRNAUCUSer were shown to exist, but the connector region comprising the truncated D loop and the extra loop was flexible. This flexibility, would enable adjustment of the mutual distance between the 3′-terminus and the anticodon consistent with that of usual tRNAs. Thus, tRNASerUCU appears to function in a similar way to that of usual tRNAs in the ribosome.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalFEBS Letters
Volume514
Issue number1
DOIs
Publication statusPublished - Mar 6 2002
Externally publishedYes

Keywords

  • Ascaris suum
  • Mitochondrion
  • Nematode
  • Nuclear magnetic resonance
  • Stable isotope
  • Unusual tRNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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