The mesenchymal factor, FGF10, initiates and maintains the outgrowth of the chick limb bud through interaction with FGF8, an apical ectodermal factor

Hideyo Ohuchi, Takashi Nakagawa, Atsuyo Yamamoto, Akihiro Araga, Takeshi Ohata, Yoshiyasu Ishimaru, Hidefumi Yoshioka, Takashi Kuwana, Tsutomu Nohno, Masahiro Yamasaki, Nobuyuki Itoh, Sumihare Noji

Research output: Contribution to journalArticle

418 Citations (Scopus)

Abstract

Vertebrate limb formation has been known to be initiated by a factor(s) secreted from the lateral plate mesoderm. In this report, we provide evidence that a member of the fibroblast growth factor (FGF) family, FGF10, emanates from the prospective limb mesoderm to serve as an endogenous initiator for limb bud formation. Fgf10 expression in the prospective limb mesenchyme precedes Fgf8 expression in the nascent apical ectoderm. Ectopic application of FGF10 to the chick embryonic flank can induce Fgf8 expression in the adjacent ectoderm, resulting in the formation of an additional complete limb. Expression of Fgf10 persists in the mesenchyme of the established limb bud and appears to interact with Fgf8 in the apical ectoderm and Sonic hedgehog in the zone of polarizing activity. These results suggest that FGF10 is a key mesenchymal factor involved in the initial budding as well as the continuous outgrowth of vertebrate limbs.

Original languageEnglish
Pages (from-to)2235-2244
Number of pages10
JournalDevelopment
Volume124
Issue number11
Publication statusPublished - Jun 1997
Externally publishedYes

Fingerprint

Limb Buds
Mesoderm
Extremities
Ectoderm
Vertebrates
Hedgehogs
Fibroblast Growth Factors

Keywords

  • Epithelial-mesenchymal interaction
  • FGF10
  • FGF8
  • Lateral plate mesoderm
  • Limb initiation

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

The mesenchymal factor, FGF10, initiates and maintains the outgrowth of the chick limb bud through interaction with FGF8, an apical ectodermal factor. / Ohuchi, Hideyo; Nakagawa, Takashi; Yamamoto, Atsuyo; Araga, Akihiro; Ohata, Takeshi; Ishimaru, Yoshiyasu; Yoshioka, Hidefumi; Kuwana, Takashi; Nohno, Tsutomu; Yamasaki, Masahiro; Itoh, Nobuyuki; Noji, Sumihare.

In: Development, Vol. 124, No. 11, 06.1997, p. 2235-2244.

Research output: Contribution to journalArticle

Ohuchi, H, Nakagawa, T, Yamamoto, A, Araga, A, Ohata, T, Ishimaru, Y, Yoshioka, H, Kuwana, T, Nohno, T, Yamasaki, M, Itoh, N & Noji, S 1997, 'The mesenchymal factor, FGF10, initiates and maintains the outgrowth of the chick limb bud through interaction with FGF8, an apical ectodermal factor', Development, vol. 124, no. 11, pp. 2235-2244.
Ohuchi, Hideyo ; Nakagawa, Takashi ; Yamamoto, Atsuyo ; Araga, Akihiro ; Ohata, Takeshi ; Ishimaru, Yoshiyasu ; Yoshioka, Hidefumi ; Kuwana, Takashi ; Nohno, Tsutomu ; Yamasaki, Masahiro ; Itoh, Nobuyuki ; Noji, Sumihare. / The mesenchymal factor, FGF10, initiates and maintains the outgrowth of the chick limb bud through interaction with FGF8, an apical ectodermal factor. In: Development. 1997 ; Vol. 124, No. 11. pp. 2235-2244.
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