The membrane topology of ALMT1, an aluminum-activated malate transport protein in wheat (Triticum aestivum)

Hirotoshi Motoda, Takayuki Sasaki, Yoshio Kano, Peter R. Ryan, Emmanuel Delhaize, Hideaki Matsumoto, Yoko Yamamoto

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The wheat ALMT1 gene encodes an aluminum (Al)-activated malate transport protein which confers Al-resistance. We investigated the membrane topology of this plasma-membrane localized protein with immunocytochemical techniques. Several green fluorescent protein (GFP)-fused and histidtne (His)-tagged chimeras of ALMT1 were prepared based on a computer-predicted secondary structure and transiently expressed in cultured mammalian cells. Antibodies raised to polypeptide epitopes of ALMT1 were used in conjunction with the antibody to the His-tags to determine the topology of ALMT1. This study shows that the ALMT1 protein contains six transmembrane domains with the amino and carboxyl termini located on the extracellular side of the plasma membrane.

Original languageEnglish
Pages (from-to)467-472
Number of pages6
JournalPlant Signaling and Behavior
Volume2
Issue number6
Publication statusPublished - Nov 2007

Fingerprint

transport proteins
malates
topology
aluminum
plasma membrane
Triticum aestivum
wheat
antibodies
chimerism
green fluorescent protein
membrane proteins
epitopes
blood proteins
polypeptides
genes
proteins
cells
methodology

Keywords

  • ALMT1
  • Aluminum resistance
  • Immunofluorescent staining
  • Malate transporter
  • Topology
  • Triticum aestivum

ASJC Scopus subject areas

  • Plant Science

Cite this

Motoda, H., Sasaki, T., Kano, Y., Ryan, P. R., Delhaize, E., Matsumoto, H., & Yamamoto, Y. (2007). The membrane topology of ALMT1, an aluminum-activated malate transport protein in wheat (Triticum aestivum). Plant Signaling and Behavior, 2(6), 467-472.

The membrane topology of ALMT1, an aluminum-activated malate transport protein in wheat (Triticum aestivum). / Motoda, Hirotoshi; Sasaki, Takayuki; Kano, Yoshio; Ryan, Peter R.; Delhaize, Emmanuel; Matsumoto, Hideaki; Yamamoto, Yoko.

In: Plant Signaling and Behavior, Vol. 2, No. 6, 11.2007, p. 467-472.

Research output: Contribution to journalArticle

Motoda, H, Sasaki, T, Kano, Y, Ryan, PR, Delhaize, E, Matsumoto, H & Yamamoto, Y 2007, 'The membrane topology of ALMT1, an aluminum-activated malate transport protein in wheat (Triticum aestivum)', Plant Signaling and Behavior, vol. 2, no. 6, pp. 467-472.
Motoda, Hirotoshi ; Sasaki, Takayuki ; Kano, Yoshio ; Ryan, Peter R. ; Delhaize, Emmanuel ; Matsumoto, Hideaki ; Yamamoto, Yoko. / The membrane topology of ALMT1, an aluminum-activated malate transport protein in wheat (Triticum aestivum). In: Plant Signaling and Behavior. 2007 ; Vol. 2, No. 6. pp. 467-472.
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