The melanocortin receptor subtypes in chicken have high preference to ACTH-derived peptides

Maria K. Ling, Eri Hotta, Zuzana Kilianova, Tatjana Haitina, Aneta Ringholm, Lisa Johansson, Nicole Gallo-Payet, Sakae Takeuchi, Helgi B. Schiöth

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Melanocortin (MC) receptors are widely distributed throughout the body of chicken, like in mammals, and participate in a wide range of physiological functions. To clarify the pharmacological impact of ligands acting in the MC system, we expressed the chicken MC1, MC2, MC3, MC4 and MC5 (cMC1-5) receptors in eukaryotic cells and performed comprehensive pharmacological characterization of the potency of endogenous and synthetic melanocortin peptides. Remarkably, the cMC receptors displayed high affinity for ACTH-derived peptides and in general low affinity for α-MSH. It is evident that not only the cMC2 receptor but also the other cMC receptors interact with ACTH-derived peptide through an epitope beyond the sequence of α-MSH. The synthetic ligand MTII was found to be a potent agonist whereas HS024 was a potent antagonist at the cMC4 receptor, indicating that these ligands are suitable for physiological studies in chicken. We also show the presence of prohormone convertase 1 (PC1) and PC2 genes in chicken, and that these peptides are coexpressed with proopiomelanocortin (POMC) in various tissues.

Original languageEnglish
Pages (from-to)626-637
Number of pages12
JournalBritish Journal of Pharmacology
Volume143
Issue number5
DOIs
Publication statusPublished - Nov 1 2004

Keywords

  • ACTH
  • GPCR
  • MC receptor subtypes
  • MSH

ASJC Scopus subject areas

  • Pharmacology

Fingerprint Dive into the research topics of 'The melanocortin receptor subtypes in chicken have high preference to ACTH-derived peptides'. Together they form a unique fingerprint.

  • Cite this

    Ling, M. K., Hotta, E., Kilianova, Z., Haitina, T., Ringholm, A., Johansson, L., Gallo-Payet, N., Takeuchi, S., & Schiöth, H. B. (2004). The melanocortin receptor subtypes in chicken have high preference to ACTH-derived peptides. British Journal of Pharmacology, 143(5), 626-637. https://doi.org/10.1038/sj.bjp.0705900