The melanocortin receptor subtypes in chicken have high preference to ACTH-derived peptides

Maria K. Ling, Eri Hotta, Zuzana Kilianova, Tatjana Haitina, Aneta Ringholm, Lisa Johansson, Nicole Gallo-Payet, Sakae Takeuchi, Helgi B. Schiöth

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Melanocortin (MC) receptors are widely distributed throughout the body of chicken, like in mammals, and participate in a wide range of physiological functions. To clarify the pharmacological impact of ligands acting in the MC system, we expressed the chicken MC1, MC2, MC3, MC4 and MC5 (cMC1-5) receptors in eukaryotic cells and performed comprehensive pharmacological characterization of the potency of endogenous and synthetic melanocortin peptides. Remarkably, the cMC receptors displayed high affinity for ACTH-derived peptides and in general low affinity for α-MSH. It is evident that not only the cMC2 receptor but also the other cMC receptors interact with ACTH-derived peptide through an epitope beyond the sequence of α-MSH. The synthetic ligand MTII was found to be a potent agonist whereas HS024 was a potent antagonist at the cMC4 receptor, indicating that these ligands are suitable for physiological studies in chicken. We also show the presence of prohormone convertase 1 (PC1) and PC2 genes in chicken, and that these peptides are coexpressed with proopiomelanocortin (POMC) in various tissues.

Original languageEnglish
Pages (from-to)626-637
Number of pages12
JournalBritish Journal of Pharmacology
Volume143
Issue number5
DOIs
Publication statusPublished - Nov 2004

Fingerprint

Melanocortin Receptors
Adrenocorticotropic Hormone
Chickens
Melanocortins
Melanocyte-Stimulating Hormones
Peptides
Ligands
Proprotein Convertase 2
Proprotein Convertase 1
Pharmacology
Pro-Opiomelanocortin
Eukaryotic Cells
Epitopes
Mammals
Genes

Keywords

  • ACTH
  • GPCR
  • MC receptor subtypes
  • MSH

ASJC Scopus subject areas

  • Pharmacology

Cite this

Ling, M. K., Hotta, E., Kilianova, Z., Haitina, T., Ringholm, A., Johansson, L., ... Schiöth, H. B. (2004). The melanocortin receptor subtypes in chicken have high preference to ACTH-derived peptides. British Journal of Pharmacology, 143(5), 626-637. https://doi.org/10.1038/sj.bjp.0705900

The melanocortin receptor subtypes in chicken have high preference to ACTH-derived peptides. / Ling, Maria K.; Hotta, Eri; Kilianova, Zuzana; Haitina, Tatjana; Ringholm, Aneta; Johansson, Lisa; Gallo-Payet, Nicole; Takeuchi, Sakae; Schiöth, Helgi B.

In: British Journal of Pharmacology, Vol. 143, No. 5, 11.2004, p. 626-637.

Research output: Contribution to journalArticle

Ling, MK, Hotta, E, Kilianova, Z, Haitina, T, Ringholm, A, Johansson, L, Gallo-Payet, N, Takeuchi, S & Schiöth, HB 2004, 'The melanocortin receptor subtypes in chicken have high preference to ACTH-derived peptides', British Journal of Pharmacology, vol. 143, no. 5, pp. 626-637. https://doi.org/10.1038/sj.bjp.0705900
Ling, Maria K. ; Hotta, Eri ; Kilianova, Zuzana ; Haitina, Tatjana ; Ringholm, Aneta ; Johansson, Lisa ; Gallo-Payet, Nicole ; Takeuchi, Sakae ; Schiöth, Helgi B. / The melanocortin receptor subtypes in chicken have high preference to ACTH-derived peptides. In: British Journal of Pharmacology. 2004 ; Vol. 143, No. 5. pp. 626-637.
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