The mechanisms and regulation of procathepsin L secretion from osteoclasts in bone resorption

Kahori Tagami, Hisao Kakegawa, Hiroshi Kamioka, Koji Sumitani, Terushige Kawata, Brigita Lenarcič, Vito Turk, Nobuhiko Katunuma

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

The secretion mechanisms of cathepsin L from osteoclasts in the process of bone resorption were investigated. The increases in bone pit numbers formed take place by PTH addition in parallel with the increases of cathepsin L and/or L-like proteinase activities in the culture medium of bone cells, and these were suppressed by the addition ofcalcitonin. The Z-Phe-Arg-MCA hydrolysing activity increased in the medium through the effect of PTH is considered to be a kind of procathepsin L by Western blotting analysis, and was suppressed by calcitonin addition. Furthermore, monensin inhibited not only the PTH-induced pit formation, but also cysteine proteinase activity in osteoclasts. Therefore, the procathepsin L excreted might be transferred from endothelial reticulum via Golgi and/or via lysosomes.

Original languageEnglish
Pages (from-to)308-312
Number of pages5
JournalFEBS Letters
Volume342
Issue number3
DOIs
Publication statusPublished - Apr 11 1994
Externally publishedYes

Keywords

  • Bone resorption
  • Calcitonin
  • Cysteine proteinase
  • Monensin
  • Parathyroid hormone
  • Procathepsin L

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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