The mechanisms and regulation of procathepsin L secretion from osteoclasts in bone resorption

Kahori Tagami; Hisao Kakegawa; Hiroshi Kamioka; Koji Sumitani; Terushige Kawata; Brigita Lenarcič; Vito Turk; Nobuhiko Katunuma

doi:10.1016/0014-5793(94)80522-9

The mechanisms and regulation of procathepsin L secretion from osteoclasts in bone resorption

Kahori Tagami, Hisao Kakegawa, Hiroshi Kamioka, Koji Sumitani, Terushige Kawata, Brigita Lenarcič, Vito Turk, Nobuhiko Katunuma

Research output: Contribution to journal › Article

27 Citations (Scopus)

Abstract

The secretion mechanisms of cathepsin L from osteoclasts in the process of bone resorption were investigated. The increases in bone pit numbers formed take place by PTH addition in parallel with the increases of cathepsin L and/or L-like proteinase activities in the culture medium of bone cells, and these were suppressed by the addition ofcalcitonin. The Z-Phe-Arg-MCA hydrolysing activity increased in the medium through the effect of PTH is considered to be a kind of procathepsin L by Western blotting analysis, and was suppressed by calcitonin addition. Furthermore, monensin inhibited not only the PTH-induced pit formation, but also cysteine proteinase activity in osteoclasts. Therefore, the procathepsin L excreted might be transferred from endothelial reticulum via Golgi and/or via lysosomes.

Original language	English
Pages (from-to)	308-312
Number of pages	5
Journal	FEBS Letters
Volume	342
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(94)80522-9
Publication status	Published - Apr 11 1994
Externally published	Yes

Keywords

Bone resorption
Calcitonin
Cysteine proteinase
Monensin
Parathyroid hormone
Procathepsin L

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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Tagami, K., Kakegawa, H., Kamioka, H., Sumitani, K., Kawata, T., Lenarcič, B., Turk, V., & Katunuma, N. (1994). The mechanisms and regulation of procathepsin L secretion from osteoclasts in bone resorption. FEBS Letters, 342(3), 308-312. https://doi.org/10.1016/0014-5793(94)80522-9

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abstract = "The secretion mechanisms of cathepsin L from osteoclasts in the process of bone resorption were investigated. The increases in bone pit numbers formed take place by PTH addition in parallel with the increases of cathepsin L and/or L-like proteinase activities in the culture medium of bone cells, and these were suppressed by the addition ofcalcitonin. The Z-Phe-Arg-MCA hydrolysing activity increased in the medium through the effect of PTH is considered to be a kind of procathepsin L by Western blotting analysis, and was suppressed by calcitonin addition. Furthermore, monensin inhibited not only the PTH-induced pit formation, but also cysteine proteinase activity in osteoclasts. Therefore, the procathepsin L excreted might be transferred from endothelial reticulum via Golgi and/or via lysosomes.",

keywords = "Bone resorption, Calcitonin, Cysteine proteinase, Monensin, Parathyroid hormone, Procathepsin L",

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AU - Tagami, Kahori

AU - Kakegawa, Hisao

AU - Kamioka, Hiroshi

AU - Sumitani, Koji

AU - Kawata, Terushige

AU - Lenarcič, Brigita

AU - Turk, Vito

AU - Katunuma, Nobuhiko

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N2 - The secretion mechanisms of cathepsin L from osteoclasts in the process of bone resorption were investigated. The increases in bone pit numbers formed take place by PTH addition in parallel with the increases of cathepsin L and/or L-like proteinase activities in the culture medium of bone cells, and these were suppressed by the addition ofcalcitonin. The Z-Phe-Arg-MCA hydrolysing activity increased in the medium through the effect of PTH is considered to be a kind of procathepsin L by Western blotting analysis, and was suppressed by calcitonin addition. Furthermore, monensin inhibited not only the PTH-induced pit formation, but also cysteine proteinase activity in osteoclasts. Therefore, the procathepsin L excreted might be transferred from endothelial reticulum via Golgi and/or via lysosomes.

AB - The secretion mechanisms of cathepsin L from osteoclasts in the process of bone resorption were investigated. The increases in bone pit numbers formed take place by PTH addition in parallel with the increases of cathepsin L and/or L-like proteinase activities in the culture medium of bone cells, and these were suppressed by the addition ofcalcitonin. The Z-Phe-Arg-MCA hydrolysing activity increased in the medium through the effect of PTH is considered to be a kind of procathepsin L by Western blotting analysis, and was suppressed by calcitonin addition. Furthermore, monensin inhibited not only the PTH-induced pit formation, but also cysteine proteinase activity in osteoclasts. Therefore, the procathepsin L excreted might be transferred from endothelial reticulum via Golgi and/or via lysosomes.

KW - Bone resorption

KW - Calcitonin

KW - Cysteine proteinase

KW - Monensin

KW - Parathyroid hormone

KW - Procathepsin L

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