The mechanism of copper leaching by intact cells of thiohacillus ferrooxidans

Tsuyoshi Sugio, Susana Flavier De Los Santos, Toru Hirose, Kenji Inagaki, Tatsuo Tano

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Under aerobic conditions, washed intact cells of Thiobacillus ferrooxidans AP19-3 solubilized copper enzymatically from a copper concentrate containing Cu(20.48%), Fe(31.61%), S(38.22%), Pb(3.84%), and Zn(4.22%). The optimum pH of copper solnbilization from copper concentrate was at pH 3.0. In contrast, under anaerobic conditions or under aerobic conditions in the presence of sodium cyanide, inhibitor of iron oxidase, the amount of copper solubilized enzymatically from the ore markedly decreased, indicating that iron oxidase is involved in the copper solubilization. Under the conditions under which iron oxidase of the cells cannot operate, Fe2+ and Cu+ ions were produced enzymatically, suggesting that a hydrogen snlfide: ferric ion oxidoreductase (SFORase) was involved in this copper solubilization from the ore. A short treatment of the strain with 0.5% phenol completely destroyed the SFORase. However, this treatment did not affect the iron oxidase of the cells. A concomitant loss of the activity of copper solnbilization from the ore was observed in 0.5% phenol-treated cells. The results strongly suggest that both iron oxidase and SFO Rase are crucial in copper ore leaching by T. ferrooxidans AP19-3.

Original languageEnglish
Pages (from-to)2293-2298
Number of pages6
JournalAgricultural and Biological Chemistry
Volume54
Issue number9
DOIs
Publication statusPublished - 1990

Fingerprint

Leaching
Copper
leaching
copper
Oxidoreductases
Iron
iron
Ores
cells
Acidithiobacillus ferrooxidans
Ions
Phenol
oxidoreductases
aerobic conditions
ions
solubilization
phenol
Sodium Cyanide
Thiobacillus
concentrates

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The mechanism of copper leaching by intact cells of thiohacillus ferrooxidans. / Sugio, Tsuyoshi; De Los Santos, Susana Flavier; Hirose, Toru; Inagaki, Kenji; Tano, Tatsuo.

In: Agricultural and Biological Chemistry, Vol. 54, No. 9, 1990, p. 2293-2298.

Research output: Contribution to journalArticle

Sugio, Tsuyoshi ; De Los Santos, Susana Flavier ; Hirose, Toru ; Inagaki, Kenji ; Tano, Tatsuo. / The mechanism of copper leaching by intact cells of thiohacillus ferrooxidans. In: Agricultural and Biological Chemistry. 1990 ; Vol. 54, No. 9. pp. 2293-2298.
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