The mechanical stimulation of cells in 3D culture within a self-assembling peptide hydrogel

Yusuke Nagai, Hidenori Yokoi, Keiko Kaihara, Keiji Naruse

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The aim of this present study was to provide a scaffold as a tool for the investigation of the effect of mechanical stimulation on three-dimensionally cultured cells. For this purpose, we developed an artificial self-assembling peptide (SPG-178) hydrogel scaffold. The structural properties of the SPG-178 peptide were confirmed by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and transmission electron microscopy (TEM). The mechanical properties of the SPG-178 hydrogel were studied using rheology measurements. The SPG-178 peptide was able to form a stable, transparent hydrogel in a neutral pH environment. In the SPG-178 hydrogel, mouse skeletal muscle cells proliferated successfully (increased by 12.4 ± 1.5 times during 8 days of incubation; mean ± SEM). When the scaffold was statically stretched, a rapid phosphorylation of ERK was observed (increased by 2.8 ± 0.2 times; mean ± SEM). These results demonstrated that the developed self-assembling peptide gel is non-cytotoxic and is a suitable tool for the investigation of the effect of mechanical stimulation on three-dimensional cell culture.

Original languageEnglish
Pages (from-to)1044-1051
Number of pages8
JournalBiomaterials
Volume33
Issue number4
DOIs
Publication statusPublished - Feb 2012

Fingerprint

Hydrogel
Hydrogels
Peptides
Scaffolds
Cells
Infrared transmission
Scanning electron microscopy
Phosphorylation
Rheology
Fourier Transform Infrared Spectroscopy
Scaffolds (biology)
Transmission Electron Microscopy
Cell culture
Muscle Cells
Fourier transform infrared spectroscopy
Muscle
Structural properties
Cultured Cells
Skeletal Muscle
Gels

Keywords

  • Cell proliferation
  • Hydrogel
  • Mechanical strain
  • Scaffold
  • Self-assembly

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

The mechanical stimulation of cells in 3D culture within a self-assembling peptide hydrogel. / Nagai, Yusuke; Yokoi, Hidenori; Kaihara, Keiko; Naruse, Keiji.

In: Biomaterials, Vol. 33, No. 4, 02.2012, p. 1044-1051.

Research output: Contribution to journalArticle

Nagai, Yusuke ; Yokoi, Hidenori ; Kaihara, Keiko ; Naruse, Keiji. / The mechanical stimulation of cells in 3D culture within a self-assembling peptide hydrogel. In: Biomaterials. 2012 ; Vol. 33, No. 4. pp. 1044-1051.
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