The major structural components of two cell surface filaments of Porphyromonas gingivalis are matured through lipoprotein precursors

Mikio Shoji, Mariko Naito, Hideharu Yukitake, Keiko Sato, Eiko Sakai, Naoya Oohara, Koji Nakayama

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Bacterial cell surface filaments play significant roles in adherence to and invasion of host cells. They are generated by the chaperone/usher pathway system (class I fimbriae), the type II secretion system (type IV pili) and the nucleation-dependent polymerization system (Curli filaments) that are categorized by their modes of expression and assembly. In this study, we found that the periodontal pathogen Porphyromonas gingivalis expressed the major structural components of two cell surface filaments (fimbrilin and the 75 kDa protein) that had extremely long prosequences in their primary gene products. N-terminal amino acid sequencing of the prosequences, treatment of P. gingivalis cells with globomycin, an inhibitor for lipoprotein-specific signal peptidase, amino acid substitution of the cysteine residue of the prosequence of fimbrilin and [3H]-palmitic acid labelling implied that fimbrilin and the 75 kDa protein were matured through their lipoprotein precursor forms. Accumulation of precursor forms of fimbrilin and the 75 kDa protein on the cell surface of the gingipain-null mutant revealed that Arg-gingipain processed these precursors on the surface to yield their mature forms, which subsequently assembled into the filamentous structures, suggesting that the transport and assembly of the major component proteins appear to be novel.

Original languageEnglish
Pages (from-to)1513-1525
Number of pages13
JournalMolecular Microbiology
Volume52
Issue number5
DOIs
Publication statusPublished - Jun 2004
Externally publishedYes

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Porphyromonas gingivalis
Cellular Structures
Lipoproteins
Proteins
Palmitic Acid
Protein Sequence Analysis
Amino Acid Substitution
Polymerization
Cysteine
Membrane Proteins
fimbrillin
Genes
Porphyromonas gingivalis argingipain

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

The major structural components of two cell surface filaments of Porphyromonas gingivalis are matured through lipoprotein precursors. / Shoji, Mikio; Naito, Mariko; Yukitake, Hideharu; Sato, Keiko; Sakai, Eiko; Oohara, Naoya; Nakayama, Koji.

In: Molecular Microbiology, Vol. 52, No. 5, 06.2004, p. 1513-1525.

Research output: Contribution to journalArticle

Shoji, Mikio ; Naito, Mariko ; Yukitake, Hideharu ; Sato, Keiko ; Sakai, Eiko ; Oohara, Naoya ; Nakayama, Koji. / The major structural components of two cell surface filaments of Porphyromonas gingivalis are matured through lipoprotein precursors. In: Molecular Microbiology. 2004 ; Vol. 52, No. 5. pp. 1513-1525.
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