The Macrophage Is a key factor in renal injuries caused by glomerular hyperf iltration

Motofumi Sasaki, Kenichi Shikata, Shinichi Okada, Satoshi Miyamoto, Shingo Nishishita, Hitomi Usui Kataoka, Chikage Sato, Jun Wada, Daisuke Ogawa, Hirofumi Makino

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Glomerular hyperflltration is a common pathway leading to glomerulosclerosis in various kinds of kidney diseases. The 5/6 renal ablation is an established experimental animal model for glomerular hyperflltration. On the other hand, low-grade inflammation is also a common mechanism for the progression of kidney diseases including diabetic nephropathy and atherosclerosis. Here we analyzed the gene expression profile in the remnant kidney tissues of 5/6 nephrectomized mice using a DNA microarray system and compared it with that of sham-operated control mice. The 5/6 nephrectomized mice showed glomerular hypertrophy and an increase in the extracellular matrix in the glomeruli. DNA microarray analysis indicated the up-regulated expression of various kinds of genes related to the inflammatory process in remnant kidneys. We confirmed the up-regulated expression of platelet factor-4, and monocyte chemoattractant protein-1, 2, and 5 in remnant kidneys by RT-PCR. The current results suggest that the inflammatory process is involved in the progression of glomerulosclerosis and is a common pathway of the pathogenesis of kidney disease.

Original languageEnglish
Pages (from-to)81-89
Number of pages9
JournalActa medica Okayama
Volume65
Issue number2
Publication statusPublished - May 12 2011

Keywords

  • Chemokine
  • Inflammation
  • Kidney

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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