The loss of outer capsid protein P2 results in nontransmissibility by the insect vector of rice dwarf phytoreovirus

Masatoshi Tomaru, Wakako Maruyama, Akira Kikuchi, Jin Yan, Yafeng Zhu, Nobuhiro Suzuki, Masamichi Isogai, Yuzuru Oguma, Ikuo Kimura, Toshihiro Omura

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24 Citations (Scopus)

Abstract

A transmission-defective (TD) isolate of rice dwarf phytoreovirus lacked the ability to infect cells when derived from the virus-free insect vector Nephotettix cincticeps. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified virus showed that among six structural proteins, the P2 outer capsid protein (encoded by genome segment S2) was absent from the TD isolate, whereas all six proteins were present in the transmission-competent (TC) isolate. P2 was not detected on immunoblots of rice plants infected with the TD isolate. Genome segment S2 and its transcript were detected in both TD and TC isolates. Sequence analysis of the S2 segment of the TD isolate revealed the presence of a termination codon due to a point mutation in the open reading frame, which might explain the absence of P2 in the TD isolate. These results demonstrate that the P2 protein is one of the factors essential for infection by the virus of vector cells and, thus, influences transmissibility by vector insects.

Original languageEnglish
Pages (from-to)8019-8023
Number of pages5
JournalJournal of Virology
Volume71
Issue number10
Publication statusPublished - Sep 26 1997
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Tomaru, M., Maruyama, W., Kikuchi, A., Yan, J., Zhu, Y., Suzuki, N., Isogai, M., Oguma, Y., Kimura, I., & Omura, T. (1997). The loss of outer capsid protein P2 results in nontransmissibility by the insect vector of rice dwarf phytoreovirus. Journal of Virology, 71(10), 8019-8023.