TY - JOUR
T1 - The lipoxygenase pathways are involved in LH-stimulated progesterone production in bovine corpus luteum
AU - Taniguchi, Hiroaki
AU - Uenoyama, Yoshihisa
AU - Miyamoto, Yoko
AU - Okuda, Kiyoshi
N1 - Funding Information:
This research was supported by Grants-in-Aid for Scientific Research (No. 11460129) and Research Fellow (No. 6032) of the Japan Society for the Promotion of Science (JSPS) from the Ministry of Education, Science, Sports and Culture of Japan. Y.M. is a research fellow by JSPS. We thank the United States Department of Agriculture, Animal Hormone Program for the gift of LH (USDA-bLH-B-6) and Dr. Katsumi Wakabayashi of Gunma University for cAMP antiserum.
PY - 2002
Y1 - 2002
N2 - We have examined the effects of endogenous lipoxygenase products on basal progesterone (P4) production by cultured bovine mid-luteal cells. The involvement of lipoxygenase products in the stimulatory effect of LH on luteal cAMP accumulation and P4 production was also examined. Bovine luteal cells from mid-cycle corpora lutea (CL) were exposed for 16 h to a lipoxygenase inhibitor (nordihydroguaiaretic acid: NDGA; 0.33-33 μM). For the last 4 h of incubation, the cells were exposed to LH and/or three different lipoxygenase products, 5-, 12- and 15-hydroxyeicosatetraenoic acid (HETE). NDGA inhibited P4 production by the cells in a dose-dependent manner (P < 0.05). NDGA-reduced P4 production was reversed by the addition of 12-HETE, but not 5- or 15-HETE, whereas 5-, 12- and 15-HETE alone showed no significant effect on P4 production in the intact cells. Furthermore, NDGA (33 μM) blocked the stimulatory action of LH on P4 production (P < 0.05), without changing cAMP accumulation (P > 0.1). When the cells were exposed to 5-, 12- or 15-HETE with LH and NDGA, only 15-HETE maintained the stimulatory effect of LH on P4 production in the cells (P < 0.05). These results suggest that endogenous lipoxygenase products play important roles in P4 production by bovine CL, i.e. basal P4 production is supported by 12-HETE, and LH-stimulated P4 production is partially mediated via the activation of lipoxygenase and subsequent 15-HETE formation downstream of the LH-activated cAMP-PKA-phosphorylation pathway.
AB - We have examined the effects of endogenous lipoxygenase products on basal progesterone (P4) production by cultured bovine mid-luteal cells. The involvement of lipoxygenase products in the stimulatory effect of LH on luteal cAMP accumulation and P4 production was also examined. Bovine luteal cells from mid-cycle corpora lutea (CL) were exposed for 16 h to a lipoxygenase inhibitor (nordihydroguaiaretic acid: NDGA; 0.33-33 μM). For the last 4 h of incubation, the cells were exposed to LH and/or three different lipoxygenase products, 5-, 12- and 15-hydroxyeicosatetraenoic acid (HETE). NDGA inhibited P4 production by the cells in a dose-dependent manner (P < 0.05). NDGA-reduced P4 production was reversed by the addition of 12-HETE, but not 5- or 15-HETE, whereas 5-, 12- and 15-HETE alone showed no significant effect on P4 production in the intact cells. Furthermore, NDGA (33 μM) blocked the stimulatory action of LH on P4 production (P < 0.05), without changing cAMP accumulation (P > 0.1). When the cells were exposed to 5-, 12- or 15-HETE with LH and NDGA, only 15-HETE maintained the stimulatory effect of LH on P4 production in the cells (P < 0.05). These results suggest that endogenous lipoxygenase products play important roles in P4 production by bovine CL, i.e. basal P4 production is supported by 12-HETE, and LH-stimulated P4 production is partially mediated via the activation of lipoxygenase and subsequent 15-HETE formation downstream of the LH-activated cAMP-PKA-phosphorylation pathway.
KW - Corpus luteum
KW - Cyclic adenosine monophosphate
KW - Luteinizing hormone
KW - Ovary
KW - Progesterone
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U2 - 10.1016/S0090-6980(01)00174-5
DO - 10.1016/S0090-6980(01)00174-5
M3 - Article
C2 - 11789897
AN - SCOPUS:0036149950
SN - 1098-8823
VL - 67
SP - 49
EP - 60
JO - Journal of Lipid Mediators
JF - Journal of Lipid Mediators
IS - 1
ER -