The leukotriene B4 receptor (BLT1) is required for effector CD8+ T cell-mediated, mast cell-dependent airway hyperresponsiveness

Christian Taube, Nobuaki Miyahara, Vanessa Ott, Brad Swanson, Katsuyuki Takeda, Joan Loader, Leonard D. Shultz, Andrew M. Tager, Andrew D. Luster, Azzeddine Dakhama, Erwin W. Gelfand

Research output: Contribution to journalArticle

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Abstract

Studies in both humans and rodents have suggested that CD8+ T cells contribute to the development of airway hyperresponsiveness (AHR) and that leukotriene B4 (LTB4) is involved in the chemotaxis of effector CD8+ T cells (TEFF) to the lung by virtue of their expression of BLT1, the receptor for LTB4. In the present study, we used a mast cell-CD8-dependent model of AHR to further define the role of BLT1 in CD8+ T cell-mediated AHR. C57BL/6+/+ and CD8-deficient (CD8-/-) mice were passively sensitized with anti-OVA IgE and exposed to OVA via the airways. Following passive sensitization and allergen exposure, C57BL/6 +/+ mice developed altered airway function, whereas passively sensitized and allergen-exposed CD8-/- mice failed to do so. CD8 -/- mice reconstituted with CD8+ TEFF developed AHR in response to challenge. In contrast, CD8-/- mice reconstituted with BLT1-deficient effector CD8+ T cells did not develop AHR. The induction of increased airway responsiveness following transfer of CD8 + TEFF or in wild-type mice could be blocked by administration of an LTB4 receptor antagonist confirming the role of BLT1 in CD8+ T cell-mediated AHR. Together, these data define the important role for mast cells and the LTB4-BLT1 pathway in the development of CD8 + T cell-mediated allergic responses in the lung.

Original languageEnglish
Pages (from-to)3157-3164
Number of pages8
JournalJournal of Immunology
Volume176
Issue number5
Publication statusPublished - Mar 1 2006
Externally publishedYes

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Leukotriene B4 Receptors
Mast Cells
T-Lymphocytes
Leukotriene B4
Allergens
Leukotriene Antagonists
Lung
Chemotaxis
Inbred C57BL Mouse
Rodentia

ASJC Scopus subject areas

  • Immunology

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The leukotriene B4 receptor (BLT1) is required for effector CD8+ T cell-mediated, mast cell-dependent airway hyperresponsiveness. / Taube, Christian; Miyahara, Nobuaki; Ott, Vanessa; Swanson, Brad; Takeda, Katsuyuki; Loader, Joan; Shultz, Leonard D.; Tager, Andrew M.; Luster, Andrew D.; Dakhama, Azzeddine; Gelfand, Erwin W.

In: Journal of Immunology, Vol. 176, No. 5, 01.03.2006, p. 3157-3164.

Research output: Contribution to journalArticle

Taube, C, Miyahara, N, Ott, V, Swanson, B, Takeda, K, Loader, J, Shultz, LD, Tager, AM, Luster, AD, Dakhama, A & Gelfand, EW 2006, 'The leukotriene B4 receptor (BLT1) is required for effector CD8+ T cell-mediated, mast cell-dependent airway hyperresponsiveness', Journal of Immunology, vol. 176, no. 5, pp. 3157-3164.
Taube, Christian ; Miyahara, Nobuaki ; Ott, Vanessa ; Swanson, Brad ; Takeda, Katsuyuki ; Loader, Joan ; Shultz, Leonard D. ; Tager, Andrew M. ; Luster, Andrew D. ; Dakhama, Azzeddine ; Gelfand, Erwin W. / The leukotriene B4 receptor (BLT1) is required for effector CD8+ T cell-mediated, mast cell-dependent airway hyperresponsiveness. In: Journal of Immunology. 2006 ; Vol. 176, No. 5. pp. 3157-3164.
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